Genetic diversity of Czech Candidatus Phytoplasma mali? strains based on multilocus gene analyses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00395034" target="_blank" >RIV/60077344:_____/13:00395034 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/25271121:_____/13:#0000477

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10658-013-0196-5" target="_blank" >http://dx.doi.org/10.1007/s10658-013-0196-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10658-013-0196-5" target="_blank" >10.1007/s10658-013-0196-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genetic diversity of Czech Candidatus Phytoplasma mali? strains based on multilocus gene analyses

Popis výsledku v původním jazyce

During 2010, samples from several apple trees were collected throughout the CR to verify identity of phytoplasmas detected in association with the apple proliferation disease. The majority of the 74 apple trees examined using molecular tools were positive for Ca. P. mali?. The 16S - 23S ribosomal genes, the ribosomal protein genes and the nitroreductase and rhodonase like genes were then studied to verify phytoplasma strain variability on multigenic bases. Two RFLP profiles and correspondingly two genetic lineages were found in the PCR-amplified fragments covering the 16S-23S rDNA spacer region. Ca. P. mali? strains belonging to rpX-A subgroup were identified in the majority of the apple tree sampled. The apple proliferation subtypes AP-15 and AT-2 exhibited nearly equal occurrence; the AT-1 subtype and a mixture of the two or all three of the AP subtypes were infrequently found. The PCR/RFLP results were confirmed by nucleotide sequence analyses of selected Ca. P. mali? strains.

Název v anglickém jazyce

Genetic diversity of Czech Candidatus Phytoplasma mali? strains based on multilocus gene analyses

Popis výsledku anglicky

During 2010, samples from several apple trees were collected throughout the CR to verify identity of phytoplasmas detected in association with the apple proliferation disease. The majority of the 74 apple trees examined using molecular tools were positive for Ca. P. mali?. The 16S - 23S ribosomal genes, the ribosomal protein genes and the nitroreductase and rhodonase like genes were then studied to verify phytoplasma strain variability on multigenic bases. Two RFLP profiles and correspondingly two genetic lineages were found in the PCR-amplified fragments covering the 16S-23S rDNA spacer region. Ca. P. mali? strains belonging to rpX-A subgroup were identified in the majority of the apple tree sampled. The apple proliferation subtypes AP-15 and AT-2 exhibited nearly equal occurrence; the AT-1 subtype and a mixture of the two or all three of the AP subtypes were infrequently found. The PCR/RFLP results were confirmed by nucleotide sequence analyses of selected Ca. P. mali? strains.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European journal of plant pathology

ISSN

0929-1873

e-ISSN

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Svazek periodika

136

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

675-688

Kód UT WoS článku

000321246300004

EID výsledku v databázi Scopus

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