Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00395560" target="_blank" >RIV/60077344:_____/13:00395560 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/13:00395560 RIV/62157124:16170/13:43871791 RIV/62157124:16810/13:43871791 RIV/60076658:12310/13:43885292

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S0031182013000486" target="_blank" >http://dx.doi.org/10.1017/S0031182013000486</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0031182013000486" target="_blank" >10.1017/S0031182013000486</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity

Popis výsledku v původním jazyce

Microscopic diagnosis of equine piroplasmoses, caused by Theileria equi and Babesia caballi, is hindered by low parasitaemia during the latent phase of the infections. However, this constraint can be overcome by the application of PCR followed by sequencing. Out of 288 animals examined, the piroplasmid DNAwas detected in 78 (271%). Multiplex PCR indicated that T. equi (188%) was more prevalent than B. caballi (73%), while mixed infections were conspicuously absent. Sequences of 69 PCR amplicons obtainedby the "catch-all" PCR were in concordance with those amplified by the multiplex strategy. Computed minimal adequate model analyses for both equine piroplasmid species separately showed a significant effect of host species and age in the case of T. equi, while in the B. caballi infections only the correlation with host sex was significant. Phylogenetic analyses inferred the occurrence of three genotypes of T. equi and B. caballi. Moreover, a novel genotype C of B. caballi was identified

Název v anglickém jazyce

Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity

Popis výsledku anglicky

Microscopic diagnosis of equine piroplasmoses, caused by Theileria equi and Babesia caballi, is hindered by low parasitaemia during the latent phase of the infections. However, this constraint can be overcome by the application of PCR followed by sequencing. Out of 288 animals examined, the piroplasmid DNAwas detected in 78 (271%). Multiplex PCR indicated that T. equi (188%) was more prevalent than B. caballi (73%), while mixed infections were conspicuously absent. Sequences of 69 PCR amplicons obtainedby the "catch-all" PCR were in concordance with those amplified by the multiplex strategy. Computed minimal adequate model analyses for both equine piroplasmid species separately showed a significant effect of host species and age in the case of T. equi, while in the B. caballi infections only the correlation with host sex was significant. Phylogenetic analyses inferred the occurrence of three genotypes of T. equi and B. caballi. Moreover, a novel genotype C of B. caballi was identified

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology

ISSN

0031-1820

e-ISSN

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Svazek periodika

140

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1096-1103

Kód UT WoS článku

000321017400005

EID výsledku v databázi Scopus

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