First report of a 16SrI-C phytoplasma infecting celery (|Apium graveolens) with stunting, bushy top and phyllody in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00396838" target="_blank" >RIV/60077344:_____/13:00396838 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/jph.12110" target="_blank" >http://dx.doi.org/10.1111/jph.12110</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jph.12110" target="_blank" >10.1111/jph.12110</a>

Jazyk výsledku

angličtina

Název v původním jazyce

First report of a 16SrI-C phytoplasma infecting celery (|Apium graveolens) with stunting, bushy top and phyllody in the Czech Republic

Popis výsledku v původním jazyce

Apium graveolens L. plants showing stunting, purplish/whitening of new leaves, flower abnormalities and bushy tops were observed in South Bohemia (Czech Republic) during 2011 and 2012. Transmission electron microscopy observations showed phytoplasmas inphloem sieve tube elements of symptomatic but not healthy plants. Polymerase chain reactions with universal and group-specific phytoplasma primers followed by restriction fragment length polymorphism analyses and sequencing of 16S rDNA enabled classification of the detected phytoplasmas into the aster yellows group, ribosomal subgroup 16SrI-C. Identical analyses of the ribosomal protein genes rpl22 and rps3 were used for further classification and revealed affiliation of the phytoplasmas with the rpIC subgroups. This is the first report of naturally occurring clover phyllody phytoplasma in A. graveolens in both the Czech Republic and worldwide.

Název v anglickém jazyce

First report of a 16SrI-C phytoplasma infecting celery (|Apium graveolens) with stunting, bushy top and phyllody in the Czech Republic

Popis výsledku anglicky

Apium graveolens L. plants showing stunting, purplish/whitening of new leaves, flower abnormalities and bushy tops were observed in South Bohemia (Czech Republic) during 2011 and 2012. Transmission electron microscopy observations showed phytoplasmas inphloem sieve tube elements of symptomatic but not healthy plants. Polymerase chain reactions with universal and group-specific phytoplasma primers followed by restriction fragment length polymorphism analyses and sequencing of 16S rDNA enabled classification of the detected phytoplasmas into the aster yellows group, ribosomal subgroup 16SrI-C. Identical analyses of the ribosomal protein genes rpl22 and rps3 were used for further classification and revealed affiliation of the phytoplasmas with the rpIC subgroups. This is the first report of naturally occurring clover phyllody phytoplasma in A. graveolens in both the Czech Republic and worldwide.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LD12074" target="_blank" >LD12074: Analýza genomových komponent fytoplazem a vývoj microarrays pro jejich detekci a management</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Phytopathology - Phytopathologische Zeitschrift

ISSN

0931-1785

e-ISSN

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Svazek periodika

161

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

666-670

Kód UT WoS článku

000322708500009

EID výsledku v databázi Scopus

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