Equine cryptosporidial infection associated with Cryptosporidium hedgehog genotype in Algeria
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00396990" target="_blank" >RIV/60077344:_____/13:00396990 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.vetpar.2013.04.041" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2013.04.041</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.vetpar.2013.04.041" target="_blank" >10.1016/j.vetpar.2013.04.041</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Equine cryptosporidial infection associated with Cryptosporidium hedgehog genotype in Algeria
Popis výsledku v původním jazyce
Faecal samples from two horse farms in Algeria keeping Arabian, Thoroughbred, and Barb horses were examined for the presence of Cryptosporidium in 2010-2011. A total of 138 faecal samples (16 from a farm keeping 50 animals and 122 from a farm with 267 horses) were screened for Cryptosporidium spp. infection using molecular tools. DNA was extracted from all samples. Nested PCR was performed to amplify fragments of the SSU rDNA and gp60 genes to determine the presence of Cryptosporidium species and genotypes. Sequence analyses of SSU and gp60 genes revealed four animals positive for the presence of subtype XIIIa A22R9 of the Cryptosporidium hedgehog genotype. The infections were not associated with diarrhoea. This study reports, for the first time, the occurrence of Cryptosporidium in Algeria and the first occurrence of the hedgehog genotype in horses. These findings support the potential role of infected horses in sylvatic-domestic transmission of Cryptosporidium.
Název v anglickém jazyce
Equine cryptosporidial infection associated with Cryptosporidium hedgehog genotype in Algeria
Popis výsledku anglicky
Faecal samples from two horse farms in Algeria keeping Arabian, Thoroughbred, and Barb horses were examined for the presence of Cryptosporidium in 2010-2011. A total of 138 faecal samples (16 from a farm keeping 50 animals and 122 from a farm with 267 horses) were screened for Cryptosporidium spp. infection using molecular tools. DNA was extracted from all samples. Nested PCR was performed to amplify fragments of the SSU rDNA and gp60 genes to determine the presence of Cryptosporidium species and genotypes. Sequence analyses of SSU and gp60 genes revealed four animals positive for the presence of subtype XIIIa A22R9 of the Cryptosporidium hedgehog genotype. The infections were not associated with diarrhoea. This study reports, for the first time, the occurrence of Cryptosporidium in Algeria and the first occurrence of the hedgehog genotype in horses. These findings support the potential role of infected horses in sylvatic-domestic transmission of Cryptosporidium.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Veterinary Parasitology
ISSN
0304-4017
e-ISSN
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Svazek periodika
197
Číslo periodika v rámci svazku
1-2
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
4
Strana od-do
350-353
Kód UT WoS článku
000324787400047
EID výsledku v databázi Scopus
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