In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00421276" target="_blank" >RIV/60077344:_____/13:00421276 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60076658:12310/13:43885518
Výsledek na webu
<a href="http://dx.doi.org/10.1111/tbed.12122" target="_blank" >http://dx.doi.org/10.1111/tbed.12122</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tbed.12122" target="_blank" >10.1111/tbed.12122</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil
Popis výsledku v původním jazyce
Ehrlichiae are obligate intracytoplasmic Gram-negative, tick-borne bacteria belonging to the Anaplasmataceae family. Ehrlichioses are considered emerging diseases in both humans and animals. Several members of the genus Ehrlichia have been isolated and propagated in vitro. This study describes the continuous propagation of a Brazilian Ehrlichia sp. isolate in IDE8 tick cells, canine DH82 cells and bovine aorta cells. Initially, the organisms were isolated from the haemolymph of a Rhipicephalus (Boophilus) microplus tick into IDE8 cells. Infected IDE8 cells were brought from Brazil to Germany, where the organisms were continuously propagated in IDE8, DH82 and bovine aorta cells. Bovine aorta cells were infected and propagated for 3months, correspondingto six subcultures, whereas the other two infected cell lines were kept for more than 1year. During the cultivation period, 36 and 14 subcultures were carried out in IDE8 and DH82 cell cultures, respectively. Reinfection of IDE8 cells wit
Název v anglickém jazyce
In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil
Popis výsledku anglicky
Ehrlichiae are obligate intracytoplasmic Gram-negative, tick-borne bacteria belonging to the Anaplasmataceae family. Ehrlichioses are considered emerging diseases in both humans and animals. Several members of the genus Ehrlichia have been isolated and propagated in vitro. This study describes the continuous propagation of a Brazilian Ehrlichia sp. isolate in IDE8 tick cells, canine DH82 cells and bovine aorta cells. Initially, the organisms were isolated from the haemolymph of a Rhipicephalus (Boophilus) microplus tick into IDE8 cells. Infected IDE8 cells were brought from Brazil to Germany, where the organisms were continuously propagated in IDE8, DH82 and bovine aorta cells. Bovine aorta cells were infected and propagated for 3months, correspondingto six subcultures, whereas the other two infected cell lines were kept for more than 1year. During the cultivation period, 36 and 14 subcultures were carried out in IDE8 and DH82 cell cultures, respectively. Reinfection of IDE8 cells wit
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Transboundary and Emerging Diseases
ISSN
1865-1674
e-ISSN
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Svazek periodika
60
Číslo periodika v rámci svazku
NOV 2013
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
7
Strana od-do
86-92
Kód UT WoS článku
000326839500013
EID výsledku v databázi Scopus
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