Laboratory bioassay assessing the enhancement of Cry3Aa toxicity to coleopteran storage pests ? preliminary results

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00423244" target="_blank" >RIV/60077344:_____/13:00423244 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Laboratory bioassay assessing the enhancement of Cry3Aa toxicity to coleopteran storage pests ? preliminary results

Popis výsledku v původním jazyce

Storage pests cause economic losses to stored grain worldwide. The traditional synthetic chemicals that have been used for their control are increasingly removed from the market because of increased regulatory constraints and insect resistance. The use of biopesticides based on Bacillus thuringiensis (Bt) spores offers a safe alternative but some storage pests show low sensitivity to any natural Cry toxin. Several recombinant proteins with N-/C-terminal and binding domain modifications of Cry3Aa were prepared in search for a derivative active on Tenebrio molitor and Tribolium castaneum (Coleoptera: Tenebrionidae). Test compounds were added to the standard larval diet and larval mortality was recorded in seven-day intervals for eight weeks of exposure.Probit analysis was used to estimate lethal effects of four doses of Cry3Aa and its derivatives and the LC50 was calculated. Slightly enhanced effects of some Cry3Aa derivatives in comparison with natural Cry3Aa toxin were detected.

Název v anglickém jazyce

Laboratory bioassay assessing the enhancement of Cry3Aa toxicity to coleopteran storage pests ? preliminary results

Popis výsledku anglicky

Storage pests cause economic losses to stored grain worldwide. The traditional synthetic chemicals that have been used for their control are increasingly removed from the market because of increased regulatory constraints and insect resistance. The use of biopesticides based on Bacillus thuringiensis (Bt) spores offers a safe alternative but some storage pests show low sensitivity to any natural Cry toxin. Several recombinant proteins with N-/C-terminal and binding domain modifications of Cry3Aa were prepared in search for a derivative active on Tenebrio molitor and Tribolium castaneum (Coleoptera: Tenebrionidae). Test compounds were added to the standard larval diet and larval mortality was recorded in seven-day intervals for eight weeks of exposure.Probit analysis was used to estimate lethal effects of four doses of Cry3Aa and its derivatives and the LC50 was calculated. Slightly enhanced effects of some Cry3Aa derivatives in comparison with natural Cry3Aa toxin were detected.

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/GAP502%2F11%2F1471" target="_blank" >GAP502/11/1471: Studium mechanismů rezistence hmyzích škůdců ke Cry toxinům Bacillus thuringiensis</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

IOBC-WPRS Bulletin (GMOs in Integrated Plant Production)

ISBN

978-92-9067-276-0

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

109-113

Název nakladatele

Federal Research Center for Cultivated Plants

Místo vydání

Darmstadt

Místo konání akce

Berlín

Datum konání akce

3. 6. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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