The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00430608" target="_blank" >RIV/60077344:_____/14:00430608 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/14:43887334

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.carres.2014.02.017" target="_blank" >http://dx.doi.org/10.1016/j.carres.2014.02.017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carres.2014.02.017" target="_blank" >10.1016/j.carres.2014.02.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick

Popis výsledku v původním jazyce

The presence of sialylated structures in tick organs was observed previously using lectin staining. Recently, we demonstrated the presence of sialylated N-glycans using mass spectrometry in tick salivary glands and the gut. However, we proposed a host (blood) origin for these glycans and mapped the transport of sialylated molecules from the gut to the salivary glands. In this report, we performed quantitation of whole sialic acid and of metabolically incorporated sialic acid (N-azido neuraminic acid) inIxodes ricinus tick samples. We show that the majority of sialylated molecules in the adult tick originate in the host (blood) and are not synthesized by the tick. Similar results were observed for tick cell cultures. The almost complete absence of ticksialylated molecules and the specific transport and localization of host structures into the tick salivary glands and the saliva raises many questions on the role of these molecules in the physiology and, specifically, the blood-feeding

Název v anglickém jazyce

The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick

Popis výsledku anglicky

The presence of sialylated structures in tick organs was observed previously using lectin staining. Recently, we demonstrated the presence of sialylated N-glycans using mass spectrometry in tick salivary glands and the gut. However, we proposed a host (blood) origin for these glycans and mapped the transport of sialylated molecules from the gut to the salivary glands. In this report, we performed quantitation of whole sialic acid and of metabolically incorporated sialic acid (N-azido neuraminic acid) inIxodes ricinus tick samples. We show that the majority of sialylated molecules in the adult tick originate in the host (blood) and are not synthesized by the tick. Similar results were observed for tick cell cultures. The almost complete absence of ticksialylated molecules and the specific transport and localization of host structures into the tick salivary glands and the saliva raises many questions on the role of these molecules in the physiology and, specifically, the blood-feeding

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carbohydrate Research

ISSN

0008-6215

e-ISSN

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Svazek periodika

389

Číslo periodika v rámci svazku

MAY 2014

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

93-99

Kód UT WoS článku

000334756600014

EID výsledku v databázi Scopus

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