Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00433365" target="_blank" >RIV/60077344:_____/14:00433365 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture
Popis výsledku v původním jazyce
In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2?3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50?60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013.
Název v anglickém jazyce
Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture
Popis výsledku anglicky
In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2?3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50?60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plant Protection Science
ISSN
1212-2580
e-ISSN
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Svazek periodika
50
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
5
Strana od-do
174-178
Kód UT WoS článku
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EID výsledku v databázi Scopus
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