Distribution of TTAGG-specific telomerase activity in insects

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00437284" target="_blank" >RIV/60077344:_____/14:00437284 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/14:43887332

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs10577-014-9436-6" target="_blank" >http://link.springer.com/article/10.1007%2Fs10577-014-9436-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10577-014-9436-6" target="_blank" >10.1007/s10577-014-9436-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distribution of TTAGG-specific telomerase activity in insects

Popis výsledku v původním jazyce

In most eukaryotes, telomeres consist of a short tandem repetitive DNA. Insect telomeres are constituted by a (TTAGG)n motif. Telomeres are maintained by telomerase, a specialized reverse transcriptase that adds this sequence to chromosome ends. We examined telomerase activity in 15 species across Insecta. We found telomerase activity in phylogenetically distant species and we conclude a distribution pattern of (TTAGG)n is generally consistent with telomerase activity. Thus, the TTAGG-telomerase systemis functional across the Insecta. Using real-time quantitative telomeric repeat amplification protocol (RTQ-TRAP), we quantified telomerase activity in different developmental stages and tissues of Periplaneta americana. Telomerase is upregulated in young instars and gradually declines during development; in adults most active in testes and ovaries. Thus, the telomerase activity of hemimetabolous insects seems to be associated with cell proliferation and organismal development.

Název v anglickém jazyce

Distribution of TTAGG-specific telomerase activity in insects

Popis výsledku anglicky

In most eukaryotes, telomeres consist of a short tandem repetitive DNA. Insect telomeres are constituted by a (TTAGG)n motif. Telomeres are maintained by telomerase, a specialized reverse transcriptase that adds this sequence to chromosome ends. We examined telomerase activity in 15 species across Insecta. We found telomerase activity in phylogenetically distant species and we conclude a distribution pattern of (TTAGG)n is generally consistent with telomerase activity. Thus, the TTAGG-telomerase systemis functional across the Insecta. Using real-time quantitative telomeric repeat amplification protocol (RTQ-TRAP), we quantified telomerase activity in different developmental stages and tissues of Periplaneta americana. Telomerase is upregulated in young instars and gradually declines during development; in adults most active in testes and ovaries. Thus, the telomerase activity of hemimetabolous insects seems to be associated with cell proliferation and organismal development.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA14-07172S" target="_blank" >GA14-07172S: Anti-stresové reakce regulující metabolickou homeostázu u hmyzu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromosome Research

ISSN

0967-3849

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

495-503

Kód UT WoS článku

000345967900005

EID výsledku v databázi Scopus

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