Validation of Flotac for the detection and quantification of Troglodytella abrassarti and Neobalantidium coli in chimpanzees and pigs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00438007" target="_blank" >RIV/60077344:_____/14:00438007 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/14:00434089 RIV/62157124:16170/14:43872668 RIV/62157124:16810/14:43872668

Výsledek na webu

<a href="http://dx.doi.org/10.1645/14-505.1" target="_blank" >http://dx.doi.org/10.1645/14-505.1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1645/14-505.1" target="_blank" >10.1645/14-505.1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Validation of Flotac for the detection and quantification of Troglodytella abrassarti and Neobalantidium coli in chimpanzees and pigs

Popis výsledku v původním jazyce

We carried out a calibration of FLOTAC for ciliates Troglodytella abrassarti and Neobalantidium coli based on the selection of a most appropriate flotation solutions, and we also tested its accuracy (i.e., number of detected stages out of known added number of stages to fecal samples) and sensitivity for trophozoites of both ciliates in chimpanzee feces and N. coli cysts in pig feces, compared the detection threshold of FLOTAC with MIF-based sedimentation, and, subsequently, tested the losses of ciliatestages during sample preparation. Nine flotation solutions were evaluated, and ZnSO4 solution (specific gravity [s.g.] 1.2) showed to be the most suitable for trophozoite detection, while Sheather's solution (s.g. 1.33) was selected as most suitable forcysts. The FLOTAC sensitivity in detection of both stages varied: for trophozoites, we found all samples were positive when the intensity of infection 10 trophozoites per gram and higher, whereas for cysts the sensitivity was lower. The

Název v anglickém jazyce

Validation of Flotac for the detection and quantification of Troglodytella abrassarti and Neobalantidium coli in chimpanzees and pigs

Popis výsledku anglicky

We carried out a calibration of FLOTAC for ciliates Troglodytella abrassarti and Neobalantidium coli based on the selection of a most appropriate flotation solutions, and we also tested its accuracy (i.e., number of detected stages out of known added number of stages to fecal samples) and sensitivity for trophozoites of both ciliates in chimpanzee feces and N. coli cysts in pig feces, compared the detection threshold of FLOTAC with MIF-based sedimentation, and, subsequently, tested the losses of ciliatestages during sample preparation. Nine flotation solutions were evaluated, and ZnSO4 solution (specific gravity [s.g.] 1.2) showed to be the most suitable for trophozoite detection, while Sheather's solution (s.g. 1.33) was selected as most suitable forcysts. The FLOTAC sensitivity in detection of both stages varied: for trophozoites, we found all samples were positive when the intensity of infection 10 trophozoites per gram and higher, whereas for cysts the sensitivity was lower. The

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Parasitology

ISSN

0022-3395

e-ISSN

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Svazek periodika

100

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

662-670

Kód UT WoS článku

000344159300015

EID výsledku v databázi Scopus

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