Phytoplasma infections in Rhododendron hybridum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00439207" target="_blank" >RIV/60077344:_____/14:00439207 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phytoplasma infections in Rhododendron hybridum

Popis výsledku v původním jazyce

The survey of the health status of azaleas and rhododendrons on production areas in the Czech Republic started in 2006. Symptoms of unknown aetiology on Rhododendron hybridum cultivar Cunningham?s White were observed in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by polymerase chain reaction using phytoplasma-specific, universal and group specific primer pairs. Restriction fragment length polymorphism analysis of 16S rDNA enabled classification of the detected phytoplasma in the aster yellows subgroup 16SrI-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma from the infected rhododendron plant (1,724 bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma.

Název v anglickém jazyce

Phytoplasma infections in Rhododendron hybridum

Popis výsledku anglicky

The survey of the health status of azaleas and rhododendrons on production areas in the Czech Republic started in 2006. Symptoms of unknown aetiology on Rhododendron hybridum cultivar Cunningham?s White were observed in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by polymerase chain reaction using phytoplasma-specific, universal and group specific primer pairs. Restriction fragment length polymorphism analysis of 16S rDNA enabled classification of the detected phytoplasma in the aster yellows subgroup 16SrI-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma from the infected rhododendron plant (1,724 bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma.

Druh

C - Kapitola v odborné knize

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LD12074" target="_blank" >LD12074: Analýza genomových komponent fytoplazem a vývoj microarrays pro jejich detekci a management</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Phytoplasmas and phytoplasma disease management: how to reduce their economic impact

ISBN

978-88-909922-0-9

Počet stran výsledku

6

Strana od-do

137-142

Počet stran knihy

287

Název nakladatele

IPWG - International Phytoplasmologist Working Group

Místo vydání

Bologna

Kód UT WoS kapitoly

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