Perturbations in dopamine synthesis lead to discrete physiological effects and impact oxidative stress response in Drosophila

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00441941" target="_blank" >RIV/60077344:_____/15:00441941 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/15:43889001

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0022191015000037" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0022191015000037</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jinsphys.2015.01.001" target="_blank" >10.1016/j.jinsphys.2015.01.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Perturbations in dopamine synthesis lead to discrete physiological effects and impact oxidative stress response in Drosophila

Popis výsledku v původním jazyce

The impact of mutations in four essential genes involved in dopamine (DA) synthesis and transport on longevity, motor behavior, and resistance to oxidative stress was monitored in Drosophila melanogaster. Median lifespans of ple2, PuZ22 and VMAT?14 mutants were significantly decreased compared to Catsup26 and wild type controls that did not significantly differ between each other. Catsup26 flies survived longer when exposed to HP or PQ compared to ple2, PuZ22 or VMAT?14 and controls. These flies also exhibited significantly higher negative geotaxis activity compared to ple2, PuZ22, VMAT?14 and controls. All mutant flies demonstrated rhythmic circadian locomotor activity in general, albeit Catsup26 and VMAT?14 flies had slightly weaker rhythms. Expression analysis of some key antioxidant genes revealed that GSTO1 expression was significantly up-regulated in all DA synthesis pathway mutants and especially in Catsup26 and VMAT?14 flies at both mRNA and protein levels. Taken together, we h

Název v anglickém jazyce

Perturbations in dopamine synthesis lead to discrete physiological effects and impact oxidative stress response in Drosophila

Popis výsledku anglicky

The impact of mutations in four essential genes involved in dopamine (DA) synthesis and transport on longevity, motor behavior, and resistance to oxidative stress was monitored in Drosophila melanogaster. Median lifespans of ple2, PuZ22 and VMAT?14 mutants were significantly decreased compared to Catsup26 and wild type controls that did not significantly differ between each other. Catsup26 flies survived longer when exposed to HP or PQ compared to ple2, PuZ22 or VMAT?14 and controls. These flies also exhibited significantly higher negative geotaxis activity compared to ple2, PuZ22, VMAT?14 and controls. All mutant flies demonstrated rhythmic circadian locomotor activity in general, albeit Catsup26 and VMAT?14 flies had slightly weaker rhythms. Expression analysis of some key antioxidant genes revealed that GSTO1 expression was significantly up-regulated in all DA synthesis pathway mutants and especially in Catsup26 and VMAT?14 flies at both mRNA and protein levels. Taken together, we h

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

<a href="/cs/project/LH14047" target="_blank" >LH14047: Hormonální řízení antistresových reakcí u hmyzu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Insect Physiology

ISSN

0022-1910

e-ISSN

—

Svazek periodika

73

Číslo periodika v rámci svazku

Feb 1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

11-19

Kód UT WoS článku

000352666300002

EID výsledku v databázi Scopus

2-s2.0-84921260303