Discrimination of phytoplasmas using an oligonucleotide microarray targeting rps3, rpl22, and rps19 genes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00443139" target="_blank" >RIV/60077344:_____/15:00443139 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cropro.2014.12.013" target="_blank" >http://dx.doi.org/10.1016/j.cropro.2014.12.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cropro.2014.12.013" target="_blank" >10.1016/j.cropro.2014.12.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Discrimination of phytoplasmas using an oligonucleotide microarray targeting rps3, rpl22, and rps19 genes

Popis výsledku v původním jazyce

Phytoplasmas are economically important pathogens of various fruit trees. Reliable techniques therefore are needed for their detection and discrimination. For this purpose, an oligonucleotide microarray targeting the ribosomal protein genes rps3, rpl22,and rps19 of phytoplasmas was tested. Following PCR (35 cycles) of total DNA from phytoplasma-infected plants with Cy5-labelled primer, the microarray reliably detected 16Sr groups I,II,III,V,VI,VII,IX,X, and XII in single infections as well as six different mixed infections (I+II,I+III, I+V, I+VII, I+IX, and I+X) prepared artificially by mixing DNA prior to PCR It also successfully classified 16Sr groups from field samples collected in the Czech Republic (16Sr groups I,III,and mixed infection I+X). Despite that it did not succeed in distinguishing another 2 artificial mixed infections (I+VI and I+XII),the microarray developed here provides a suitable alternative to rRNA-based microarrays published previously and where only single infec

Název v anglickém jazyce

Discrimination of phytoplasmas using an oligonucleotide microarray targeting rps3, rpl22, and rps19 genes

Popis výsledku anglicky

Phytoplasmas are economically important pathogens of various fruit trees. Reliable techniques therefore are needed for their detection and discrimination. For this purpose, an oligonucleotide microarray targeting the ribosomal protein genes rps3, rpl22,and rps19 of phytoplasmas was tested. Following PCR (35 cycles) of total DNA from phytoplasma-infected plants with Cy5-labelled primer, the microarray reliably detected 16Sr groups I,II,III,V,VI,VII,IX,X, and XII in single infections as well as six different mixed infections (I+II,I+III, I+V, I+VII, I+IX, and I+X) prepared artificially by mixing DNA prior to PCR It also successfully classified 16Sr groups from field samples collected in the Czech Republic (16Sr groups I,III,and mixed infection I+X). Despite that it did not succeed in distinguishing another 2 artificial mixed infections (I+VI and I+XII),the microarray developed here provides a suitable alternative to rRNA-based microarrays published previously and where only single infec

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Crop Protection

ISSN

0261-2194

e-ISSN

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Svazek periodika

70

Číslo periodika v rámci svazku

January 2015

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

47-52

Kód UT WoS článku

000350088200007

EID výsledku v databázi Scopus

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