Evalution of the diet of a carabid predator using fluorescent marking of prey

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00446125" target="_blank" >RIV/60077344:_____/15:00446125 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.eje.cz/pdfs/eje/2015/03/11.pdf" target="_blank" >http://www.eje.cz/pdfs/eje/2015/03/11.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14411/eje.2015.054" target="_blank" >10.14411/eje.2015.054</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evalution of the diet of a carabid predator using fluorescent marking of prey

Popis výsledku v původním jazyce

We present a UV-fluorescent dye-based prey marking technique, using three different dyes, with later detection of the dyes in the guts of predators using a micro-plate fluorescent reader. By using this new method of detecting dyes based on their different excitation and emission characteristics it is possible to simultaneously screen 96 samples for multiple fluorescent-markers in less than 30 minutes. The marking is stable, cheap, non-toxic and had no effect on the choice of the carabid predator Pterostichus melanarius between marked and unmarked fly larvae (Musca domestica). Different fluorescent dyes provide variable detection intervals up to 100% detectability at 96 h post-ingestion by this predator. The simple marking, extraction and detection methods presented could be used in food web research to map individual trophic links and predator preference for different types of prey.

Název v anglickém jazyce

Evalution of the diet of a carabid predator using fluorescent marking of prey

Popis výsledku anglicky

We present a UV-fluorescent dye-based prey marking technique, using three different dyes, with later detection of the dyes in the guts of predators using a micro-plate fluorescent reader. By using this new method of detecting dyes based on their different excitation and emission characteristics it is possible to simultaneously screen 96 samples for multiple fluorescent-markers in less than 30 minutes. The marking is stable, cheap, non-toxic and had no effect on the choice of the carabid predator Pterostichus melanarius between marked and unmarked fly larvae (Musca domestica). Different fluorescent dyes provide variable detection intervals up to 100% detectability at 96 h post-ingestion by this predator. The simple marking, extraction and detection methods presented could be used in food web research to map individual trophic links and predator preference for different types of prey.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Entomology

ISSN

1210-5759

e-ISSN

—

Svazek periodika

112

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

477-485

Kód UT WoS článku

000360715400011

EID výsledku v databázi Scopus

2-s2.0-84942863783