Monitoring of Multilayered Bacterial Biofilm Morphology by Cryo-SEM for Raman Spectroscopy Measurements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00450101" target="_blank" >RIV/60077344:_____/15:00450101 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081731:_____/15:00450101 RIV/00216305:26210/15:PU115129

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of Multilayered Bacterial Biofilm Morphology by Cryo-SEM for Raman Spectroscopy Measurements

Popis výsledku v původním jazyce

Staphylococcus epidermidis has been recently recognized as an important cause of serious biofilm infections associated with implanted medical devices. In presented work the multi-layered biofilms formed by these microorganisms were observed by scanning electron microscope (SEM), in particular with using freeze-fracturing technique. The freeze-fracture technique consists of physically breaking apart (fracturing) a rapidly frozen biological sample; structural detail exposed by the fracture plane is then visualized by metal deposition. An optional step, involving vacuum sublimation of ice, may be carried out after fracturing. Freeze fracture is unique among electron microscopic techniques in providing planar views of the internal organization of membranesor biofilms. Deep etching of ultra-rapidly frozen samples permits visualization of the surface structure of cells and their components. Our sample was fractured after rapid-freezing into liquid nitrogen, the air-water was removed by shor

Název v anglickém jazyce

Monitoring of Multilayered Bacterial Biofilm Morphology by Cryo-SEM for Raman Spectroscopy Measurements

Popis výsledku anglicky

Staphylococcus epidermidis has been recently recognized as an important cause of serious biofilm infections associated with implanted medical devices. In presented work the multi-layered biofilms formed by these microorganisms were observed by scanning electron microscope (SEM), in particular with using freeze-fracturing technique. The freeze-fracture technique consists of physically breaking apart (fracturing) a rapidly frozen biological sample; structural detail exposed by the fracture plane is then visualized by metal deposition. An optional step, involving vacuum sublimation of ice, may be carried out after fracturing. Freeze fracture is unique among electron microscopic techniques in providing planar views of the internal organization of membranesor biofilms. Deep etching of ultra-rapidly frozen samples permits visualization of the surface structure of cells and their components. Our sample was fractured after rapid-freezing into liquid nitrogen, the air-water was removed by shor

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

S3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

2

Strana od-do

187-188

Kód UT WoS článku

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EID výsledku v databázi Scopus

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