Expression profiling of circulating tumor cells in metastatic breast cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00450240" target="_blank" >RIV/60077344:_____/15:00450240 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10549-014-3215-0" target="_blank" >http://dx.doi.org/10.1007/s10549-014-3215-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10549-014-3215-0" target="_blank" >10.1007/s10549-014-3215-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression profiling of circulating tumor cells in metastatic breast cancer

Popis výsledku v původním jazyce

Circulating tumor cells (CTCs) are prognostic in all stages of breast cancer. However, since they are extremely rare, little is known about the molecular nature of these cells. We report a novel strategy for the isolation and expression profiling of purepopulations of CTCs derived from peripheral blood. We developed a method to isolate CTCs based on immunomagnetic capture followed by fluorescence-activated cell sorting (IE/FACS). After assay validation using the BT474 cell line spiked into blood samples in vitro, RNA from CTCs isolated from the blood of five metastatic breast cancer (MBC) patients was linearly amplified and subjected to gene expression profiling via cDNA microarrays. We isolated a range of 9-993 captured CTCs from five MBC patients' blood and profiled their RNA in comparison to a diverse panel of primary breast tumors (n = 55). Differential expression analysis revealed CTCs to have downregulated apoptosis.

Název v anglickém jazyce

Expression profiling of circulating tumor cells in metastatic breast cancer

Popis výsledku anglicky

Circulating tumor cells (CTCs) are prognostic in all stages of breast cancer. However, since they are extremely rare, little is known about the molecular nature of these cells. We report a novel strategy for the isolation and expression profiling of purepopulations of CTCs derived from peripheral blood. We developed a method to isolate CTCs based on immunomagnetic capture followed by fluorescence-activated cell sorting (IE/FACS). After assay validation using the BT474 cell line spiked into blood samples in vitro, RNA from CTCs isolated from the blood of five metastatic breast cancer (MBC) patients was linearly amplified and subjected to gene expression profiling via cDNA microarrays. We isolated a range of 9-993 captured CTCs from five MBC patients' blood and profiled their RNA in comparison to a diverse panel of primary breast tumors (n = 55). Differential expression analysis revealed CTCs to have downregulated apoptosis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Breast Cancer Research and Treatment

ISSN

0167-6806

e-ISSN

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Svazek periodika

149

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

121-131

Kód UT WoS článku

000348183700013

EID výsledku v databázi Scopus

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