Phylogenetic and chemical studies in the potential psychotropic species complex of Psilocybe atrobrunnea with taxonomic and nomenclatural notes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00453472" target="_blank" >RIV/60077344:_____/15:00453472 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985831:_____/15:00453472 RIV/61388971:_____/15:00453472 RIV/60076658:12310/15:43890323

Výsledek na webu

<a href="http://dx.doi.org/10.3767/003158515X685283" target="_blank" >http://dx.doi.org/10.3767/003158515X685283</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3767/003158515X685283" target="_blank" >10.3767/003158515X685283</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phylogenetic and chemical studies in the potential psychotropic species complex of Psilocybe atrobrunnea with taxonomic and nomenclatural notes

Popis výsledku v původním jazyce

Five Psilocybe species with unresolved systematic position (P. atrobrunnea, P. laetissima, P. medullosa, P. pelliculosa, and P. silvatica) were investigated using four molecular markers (EF1-?, ITS, LSU, and IGS). Phylogenetic analysis revealed that withthe exception of P. laetissima, which is now rightfully classified in the genus Leratiomyces, all investigated species belong to Psilocybe sect. Psilocybe. For the first time, psychotropic compounds psilocin and psilocybin were detected in P. medullosausing gas chromatography-mass spectrometry. On the contrary, neither psilocin, nor psilocybin was detected in P. atrobrunnea and negative results were also obtained from mycelia grown in vitro on tryptamine/tryptophan-amended media. These results strongly suggest that biosynthesis of these alkaloids was lost in P. atrobrunnea. With the exception of minor differences detected in EF1-? marker, all sequences of American and European collections of P. atrobrunnea were identical. On the other

Název v anglickém jazyce

Phylogenetic and chemical studies in the potential psychotropic species complex of Psilocybe atrobrunnea with taxonomic and nomenclatural notes

Popis výsledku anglicky

Five Psilocybe species with unresolved systematic position (P. atrobrunnea, P. laetissima, P. medullosa, P. pelliculosa, and P. silvatica) were investigated using four molecular markers (EF1-?, ITS, LSU, and IGS). Phylogenetic analysis revealed that withthe exception of P. laetissima, which is now rightfully classified in the genus Leratiomyces, all investigated species belong to Psilocybe sect. Psilocybe. For the first time, psychotropic compounds psilocin and psilocybin were detected in P. medullosausing gas chromatography-mass spectrometry. On the contrary, neither psilocin, nor psilocybin was detected in P. atrobrunnea and negative results were also obtained from mycelia grown in vitro on tryptamine/tryptophan-amended media. These results strongly suggest that biosynthesis of these alkaloids was lost in P. atrobrunnea. With the exception of minor differences detected in EF1-? marker, all sequences of American and European collections of P. atrobrunnea were identical. On the other

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Persoonia

ISSN

0031-5850

e-ISSN

—

Svazek periodika

34

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

000358183400001

EID výsledku v databázi Scopus

2-s2.0-84937552252