Respiratory metabolism of salivary glands during the late larval and prepupal development of Drosophila melanogaster

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00462352" target="_blank" >RIV/60077344:_____/15:00462352 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0022191015001328" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0022191015001328</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jinsphys.2015.06.013" target="_blank" >10.1016/j.jinsphys.2015.06.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Respiratory metabolism of salivary glands during the late larval and prepupal development of Drosophila melanogaster

Popis výsledku v původním jazyce

During the late larval period, the salivary glands (SG) of Drosophila show a cascade of cytological changes associated with exocytosis and the expectoration of the proteinaceous glue that is used to affix the pupariating larva to a substrate. After puparium formation (APF), SG undergo extensive cytoplasmic vacuolation due to endocytosis, vacuole consolidation and massive apocrine secretion. Here we investigated possible correlations between cytological changes, the puffing pattern in polytene chromosomes and respiratory metabolism of the SG. The carefully staged SG were explanted into small amounts (1 or 2 mu l) of tissue culture medium. The respiratory metabolism of single or up to 3 pairs of glands was evaluated by recording the rate of O-2 consumption using a scanning microrespirographic technique sensitive to sub-nanoliter volumes of the respiratory O-2 or CO2. The recordings were carried out at times between 8 h before pupariation (BPF), until 16 h APF, at which point the SG completely disintegrate. At the early wandering larval stage (8 h BPF), the glands consume 2 nl of O-2/gland/min (=2500 mu l O-2/g/h). This relatively high metabolic rate decreases down to 1.2-1.3 nl of O-2 during the endogenous peak in ecdysteroid concentration that culminates around pupariation. The metabolic decline coincides with the exocytosis of the proteinaceous glue. During and shortly after puparium formation, which is accompanied cytologically by intense vacuolation, O-2 consumption in the SG temporarily increases to 1.6 nl O-2/gland/min. After this time, the metabolic rate of the SG decreases downward steadily until 16 h APF, when the glands disintegrate and cease to consume oxygen. The SG we analyzed from Drosophila larvae were composed of 134 intrinsic cells, with the average volume of one lobe being 37 nl.

Název v anglickém jazyce

Respiratory metabolism of salivary glands during the late larval and prepupal development of Drosophila melanogaster

Popis výsledku anglicky

During the late larval period, the salivary glands (SG) of Drosophila show a cascade of cytological changes associated with exocytosis and the expectoration of the proteinaceous glue that is used to affix the pupariating larva to a substrate. After puparium formation (APF), SG undergo extensive cytoplasmic vacuolation due to endocytosis, vacuole consolidation and massive apocrine secretion. Here we investigated possible correlations between cytological changes, the puffing pattern in polytene chromosomes and respiratory metabolism of the SG. The carefully staged SG were explanted into small amounts (1 or 2 mu l) of tissue culture medium. The respiratory metabolism of single or up to 3 pairs of glands was evaluated by recording the rate of O-2 consumption using a scanning microrespirographic technique sensitive to sub-nanoliter volumes of the respiratory O-2 or CO2. The recordings were carried out at times between 8 h before pupariation (BPF), until 16 h APF, at which point the SG completely disintegrate. At the early wandering larval stage (8 h BPF), the glands consume 2 nl of O-2/gland/min (=2500 mu l O-2/g/h). This relatively high metabolic rate decreases down to 1.2-1.3 nl of O-2 during the endogenous peak in ecdysteroid concentration that culminates around pupariation. The metabolic decline coincides with the exocytosis of the proteinaceous glue. During and shortly after puparium formation, which is accompanied cytologically by intense vacuolation, O-2 consumption in the SG temporarily increases to 1.6 nl O-2/gland/min. After this time, the metabolic rate of the SG decreases downward steadily until 16 h APF, when the glands disintegrate and cease to consume oxygen. The SG we analyzed from Drosophila larvae were composed of 134 intrinsic cells, with the average volume of one lobe being 37 nl.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Insect Physiology

ISSN

0022-1910

e-ISSN

—

Svazek periodika

81

Číslo periodika v rámci svazku

October 01

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

109-117

Kód UT WoS článku

000361860300014

EID výsledku v databázi Scopus

2-s2.0-84937204119