Multigene Sequence Analysis of Aster Yellows Phytoplasma Associated with Primrose Yellows
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00459073" target="_blank" >RIV/60077344:_____/16:00459073 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1111/jph.12442" target="_blank" >http://dx.doi.org/10.1111/jph.12442</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jph.12442" target="_blank" >10.1111/jph.12442</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Multigene Sequence Analysis of Aster Yellows Phytoplasma Associated with Primrose Yellows
Popis výsledku v původním jazyce
Primula acaulis (L.) Hill. plants showing stunting, leaf-yellowing and virescence were first discovered in the Czech Republic. Polymerase chain reactions with subsequent restriction fragment length polymorphism analyses and sequencing enabled classification of the detected phytoplasmas into the aster yellows group, ribosomal subgroup 16SrI-B, tufI-B, rpI-B, groELIB-III and SecY-IB subgroups. Phylogeny of the 16S rRNA gene sequences as well as sequence analysis of several chromosomal regions, such as the 16S-23S ribosomal operon, ribosomal proteins, spc ribosomal protein operon, genes for elongation factor EF-Tu, molecular chaperonin large subunit GroEL, immunodominant membrane protein, ribosome recycling factor, urydilate kinase, ATP- and Zn2+-dependent proteases not only confirmed its affiliation with the Candidatus Phytoplasma asteris' species but also enabled its detailed molecular characterization. The less researched regions of phytoplasma genome (amp, adk, hflB, pyrH-frr genes) could be valuable as additional markers for phytoplasma through differentiation especially within the 16SrI-B ribosomal subgroup.
Název v anglickém jazyce
Multigene Sequence Analysis of Aster Yellows Phytoplasma Associated with Primrose Yellows
Popis výsledku anglicky
Primula acaulis (L.) Hill. plants showing stunting, leaf-yellowing and virescence were first discovered in the Czech Republic. Polymerase chain reactions with subsequent restriction fragment length polymorphism analyses and sequencing enabled classification of the detected phytoplasmas into the aster yellows group, ribosomal subgroup 16SrI-B, tufI-B, rpI-B, groELIB-III and SecY-IB subgroups. Phylogeny of the 16S rRNA gene sequences as well as sequence analysis of several chromosomal regions, such as the 16S-23S ribosomal operon, ribosomal proteins, spc ribosomal protein operon, genes for elongation factor EF-Tu, molecular chaperonin large subunit GroEL, immunodominant membrane protein, ribosome recycling factor, urydilate kinase, ATP- and Zn2+-dependent proteases not only confirmed its affiliation with the Candidatus Phytoplasma asteris' species but also enabled its detailed molecular characterization. The less researched regions of phytoplasma genome (amp, adk, hflB, pyrH-frr genes) could be valuable as additional markers for phytoplasma through differentiation especially within the 16SrI-B ribosomal subgroup.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Phytopathology - Phytopathologische Zeitschrift
ISSN
0931-1785
e-ISSN
—
Svazek periodika
164
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
11
Strana od-do
166-176
Kód UT WoS článku
000370141200003
EID výsledku v databázi Scopus
2-s2.0-84958957303