First report and comparative study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its symbiont bacteria from subcontinental India

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00474276" target="_blank" >RIV/60077344:_____/17:00474276 - isvavai.cz</a>

Výsledek na webu

<a href="http://journals.fcla.edu/jon/article/view/93305" target="_blank" >http://journals.fcla.edu/jon/article/view/93305</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

First report and comparative study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its symbiont bacteria from subcontinental India

Popis výsledku v původním jazyce

Two populations (CS19 and CS20) of entomopathogenic nematodes were isolated from the soils of vegetable fields from Bijnor district, India. Based on morphological, morphometrical, and molecular studies, the nematodes were identified as Steinernema surkhetense. This work represents the first report of this species in India. The infective juveniles (IJs) showed morphometrical and morphological differences, with the original description based on longer IJs size. The IJs of the Indian isolates possess six ridges in their lateral field instead of eight reported in the original description. The analysis of ITS-rDNA sequences revealed nucleotide differences at 345, 608, and 920 positions in aligned data. No difference was observed in D2-D3 domain. The S. surkhetense COI gene was studied for the first time as well as the molecular characterization of their Xenorhabdus symbiont using the sequences of recA and gyrB genes revealing Xenorhabdus stockiae as its symbiont. These data, together with the finding of X. stockiae, suggest that this bacterium is widespread among South Asian nematodes from the ‘‘carpocapsae’’ group. Virulence of both isolates was tested on Spodoptera litura. The strain CS19 was capable to kill the larvae with 31.78 IJs at 72 hr, whereas CS20 needed 67.7 IJs

Název v anglickém jazyce

First report and comparative study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its symbiont bacteria from subcontinental India

Popis výsledku anglicky

Two populations (CS19 and CS20) of entomopathogenic nematodes were isolated from the soils of vegetable fields from Bijnor district, India. Based on morphological, morphometrical, and molecular studies, the nematodes were identified as Steinernema surkhetense. This work represents the first report of this species in India. The infective juveniles (IJs) showed morphometrical and morphological differences, with the original description based on longer IJs size. The IJs of the Indian isolates possess six ridges in their lateral field instead of eight reported in the original description. The analysis of ITS-rDNA sequences revealed nucleotide differences at 345, 608, and 920 positions in aligned data. No difference was observed in D2-D3 domain. The S. surkhetense COI gene was studied for the first time as well as the molecular characterization of their Xenorhabdus symbiont using the sequences of recA and gyrB genes revealing Xenorhabdus stockiae as its symbiont. These data, together with the finding of X. stockiae, suggest that this bacterium is widespread among South Asian nematodes from the ‘‘carpocapsae’’ group. Virulence of both isolates was tested on Spodoptera litura. The strain CS19 was capable to kill the larvae with 31.78 IJs at 72 hr, whereas CS20 needed 67.7 IJs

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10613 - Zoology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Nematology

ISSN

0022-300X

e-ISSN

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Svazek periodika

49

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

92-102

Kód UT WoS článku

000402911300010

EID výsledku v databázi Scopus

2-s2.0-85019931805