Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00475314" target="_blank" >RIV/60077344:_____/17:00475314 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/17:00475314 RIV/60076658:12310/17:43895799

Výsledek na webu

<a href="http://dx.doi.org/10.1111/1744-7917.12314" target="_blank" >http://dx.doi.org/10.1111/1744-7917.12314</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1744-7917.12314" target="_blank" >10.1111/1744-7917.12314</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut

Popis výsledku v původním jazyce

This study examined the biochemical characteristics of alpha-amylase and hormonal (adipokinetic hormone: AKH) stimulation of alpha-amylase activity in the cockroach (Periplaneta americana) midgut. We applied two AKHs in vivo and in vitro, then measured resultant amylase activity and gene expression, as well as the expression of AKH receptor (AKHR). The results revealed that optimal amylase activity is characterized by the following: pH: 5.7, temperature: 38.4 degrees C, K-m (Michaelis-Menten constant): 2.54 mg starch/mL, and V-max (maximum reaction velocity): 0.185 mu mol maltose/mL/min. In vivo application of AKHs resulted in significant increase of amylase activity: by two-fold in the gastric caeca and 4-7 fold in the rest of the midgut. In vitro experiments supported results seen in vivo: a 24-h incubation with the hormones resulted in the increase of amylase activity by 1.4 times in the caeca and 4-9 times in the midgut. Further, gene expression analyses reveal that AKHR is expressed in both the caeca and the rest of the midgut, although expression levels in the former were 23 times higher than levels in the latter. A similar pattern was found for the amylase (AMY) gene. Hormonal treatment did not affect the expression of either gene. This study is the first to provide evidence indicating direct AKH stimulation of digestive enzyme activity in the insect midgut, supported by specific AKHR gene expression in this organ.

Název v anglickém jazyce

Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut

Popis výsledku anglicky

This study examined the biochemical characteristics of alpha-amylase and hormonal (adipokinetic hormone: AKH) stimulation of alpha-amylase activity in the cockroach (Periplaneta americana) midgut. We applied two AKHs in vivo and in vitro, then measured resultant amylase activity and gene expression, as well as the expression of AKH receptor (AKHR). The results revealed that optimal amylase activity is characterized by the following: pH: 5.7, temperature: 38.4 degrees C, K-m (Michaelis-Menten constant): 2.54 mg starch/mL, and V-max (maximum reaction velocity): 0.185 mu mol maltose/mL/min. In vivo application of AKHs resulted in significant increase of amylase activity: by two-fold in the gastric caeca and 4-7 fold in the rest of the midgut. In vitro experiments supported results seen in vivo: a 24-h incubation with the hormones resulted in the increase of amylase activity by 1.4 times in the caeca and 4-9 times in the midgut. Further, gene expression analyses reveal that AKHR is expressed in both the caeca and the rest of the midgut, although expression levels in the former were 23 times higher than levels in the latter. A similar pattern was found for the amylase (AMY) gene. Hormonal treatment did not affect the expression of either gene. This study is the first to provide evidence indicating direct AKH stimulation of digestive enzyme activity in the insect midgut, supported by specific AKHR gene expression in this organ.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA14-07172S" target="_blank" >GA14-07172S: Anti-stresové reakce regulující metabolickou homeostázu u hmyzu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Insect Science

ISSN

1672-9609

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

259-269

Kód UT WoS článku

000398603200008

EID výsledku v databázi Scopus

2-s2.0-84962641165