Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00478939" target="_blank" >RIV/60077344:_____/17:00478939 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.bioscience.org/2017/v22/af/4574/list.htm" target="_blank" >https://www.bioscience.org/2017/v22/af/4574/list.htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2741/4574" target="_blank" >10.2741/4574</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum

Popis výsledku v původním jazyce

The obligate intracellular pathogen Anaplasma phagocytophilum infects vertebrate and tick hosts. In this study, a genome-wide search for cytoskeleton components was performed in the tick vector, Ixodes scapularis. The available transcriptomics and proteomics data was then used to characterize the mRNA and protein levels of I. scapularis cytoskeleton components in response to A. phagocytophilum infection. The results showed that cytoskeleton components described in other model organisms were present in the I. scapularis genome. One type of intermediate filaments (lamin), a family of septins that was recently implicated in the cellular response to intracellular pathogens, and several members of motor proteins (kinesins and dyneins) that could be implicated in the cytoplasmic movements of A. phagocytophilum were found. The results showed that levels of tubulin, actin, septin, actin-related proteins and motor proteins were affected by A. phagocytophilum, probably to facilitate infection in I. scapularis. Functional studies demonstrated a role for selected cytoskeleton components in pathogen infection. These results provided a more comprehensive view of the cytoskeletal components involved in the response to A. phagocytophilum infection in ticks.

Název v anglickém jazyce

Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum

Popis výsledku anglicky

The obligate intracellular pathogen Anaplasma phagocytophilum infects vertebrate and tick hosts. In this study, a genome-wide search for cytoskeleton components was performed in the tick vector, Ixodes scapularis. The available transcriptomics and proteomics data was then used to characterize the mRNA and protein levels of I. scapularis cytoskeleton components in response to A. phagocytophilum infection. The results showed that cytoskeleton components described in other model organisms were present in the I. scapularis genome. One type of intermediate filaments (lamin), a family of septins that was recently implicated in the cellular response to intracellular pathogens, and several members of motor proteins (kinesins and dyneins) that could be implicated in the cytoplasmic movements of A. phagocytophilum were found. The results showed that levels of tubulin, actin, septin, actin-related proteins and motor proteins were affected by A. phagocytophilum, probably to facilitate infection in I. scapularis. Functional studies demonstrated a role for selected cytoskeleton components in pathogen infection. These results provided a more comprehensive view of the cytoskeletal components involved in the response to A. phagocytophilum infection in ticks.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Bioscience-Landmark

ISSN

1093-4715

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

Jun 1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

1830-1844

Kód UT WoS článku

000439039200001

EID výsledku v databázi Scopus

2-s2.0-85027704584