RSM22, mtYsxC and PNKD-like proteins are required for mitochondrial translation in Trypanosoma brucei

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00479262" target="_blank" >RIV/60077344:_____/17:00479262 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/17:43895457

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.mito.2017.01.003" target="_blank" >http://dx.doi.org/10.1016/j.mito.2017.01.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mito.2017.01.003" target="_blank" >10.1016/j.mito.2017.01.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

RSM22, mtYsxC and PNKD-like proteins are required for mitochondrial translation in Trypanosoma brucei

Popis výsledku v původním jazyce

Mitochondrial ribosomes evolved from prokaryotic ribosomes, with which they therefore share more common features than with their counterparts in the cytosol. Yet, mitochondrial ribosomes are highly diverse in structure and composition, having undergone considerable changes, including reduction of their RNA component and varying degree of acquisition of novel proteins in various phylogenetic lineages. Here, we present functional analysis of three putative mitochondrial ribosome-associated proteins (RSM22, mtYsxC and PNKD-like) in Trypanosoma brucei, originally identified by database mining. While in other systems the homologs of RSM22 are known as components of mitochondria ribosomes, YsxC was linked with ribosomes only in bacteria. The PNKD-like protein shows similarity to a human protein, the defects of which cause PNKD (paroxysmal nonkinesigenic dyskinesia). Here we show that all three proteins are important for the growth of T. brucei. They play an important function in mitochondrial translation, as their ablation by RNAi rapidly and severely affected the de novo synthesis of mitochondrial proteins. Moreover, following the RNAi-mediated depletion of RSM22, structure of the small subunit of mitochondrial ribosome becomes severely compromised, suggesting a role of RSM22 in ribosomal assembly and/or stability. (C) 2017 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

Název v anglickém jazyce

RSM22, mtYsxC and PNKD-like proteins are required for mitochondrial translation in Trypanosoma brucei

Popis výsledku anglicky

Mitochondrial ribosomes evolved from prokaryotic ribosomes, with which they therefore share more common features than with their counterparts in the cytosol. Yet, mitochondrial ribosomes are highly diverse in structure and composition, having undergone considerable changes, including reduction of their RNA component and varying degree of acquisition of novel proteins in various phylogenetic lineages. Here, we present functional analysis of three putative mitochondrial ribosome-associated proteins (RSM22, mtYsxC and PNKD-like) in Trypanosoma brucei, originally identified by database mining. While in other systems the homologs of RSM22 are known as components of mitochondria ribosomes, YsxC was linked with ribosomes only in bacteria. The PNKD-like protein shows similarity to a human protein, the defects of which cause PNKD (paroxysmal nonkinesigenic dyskinesia). Here we show that all three proteins are important for the growth of T. brucei. They play an important function in mitochondrial translation, as their ablation by RNAi rapidly and severely affected the de novo synthesis of mitochondrial proteins. Moreover, following the RNAi-mediated depletion of RSM22, structure of the small subunit of mitochondrial ribosome becomes severely compromised, suggesting a role of RSM22 in ribosomal assembly and/or stability. (C) 2017 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/GA15-21974S" target="_blank" >GA15-21974S: Studium vztahů mezi RNA-vážícími bílkovinami a mitochondriálním genomem trypanosom</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mitochondrion

ISSN

1567-7249

e-ISSN

—

Svazek periodika

34

Číslo periodika v rámci svazku

MAY

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

67-74

Kód UT WoS článku

000402023900010

EID výsledku v databázi Scopus

2-s2.0-85009952092