Testing parasiticidal and immunomodulatory substances to combat myxozoan infections in aquaculture using novel in vitro and in vivo models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00488261" target="_blank" >RIV/60077344:_____/17:00488261 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Testing parasiticidal and immunomodulatory substances to combat myxozoan infections in aquaculture using novel in vitro and in vivo models

Popis výsledku v původním jazyce

In the initiation phase of the project we reared and/or acquired approx. 3000 specific pathogen-free carp for experimental use. We used these fish to perform repeated intraperitoneal infection challenges with S. molnari blood stages and to monitor parasite proliferation over time using qPCR. Based on maximum parasite numbers in the blood, we have now established a continuous in vivo production line for parasite blood stages every 28 days (G2 – technically realized result/technicky realizované výsledky – prototyp, funkční vzorek) which allows us to be independent from the collection of environmental samples which is especially hindered in the winter months (frozen ponds, low temperature and undetectable parasite loads). Blood stages are harvested by centrifugation and used for in vivo and in vitro trials. We have performed a hematological study on carp blood and developed an immunoglobulin M sec qPCR assay to determine the immunological response of carp to parasite infection (in preparation for publication). Furthermore, together with Skretting ARC and Lallemond Animal Nutrition we have composed recipes for two carp diets, one based on curcumin (parasiticidal substance) and one based on yeast and probiotics (immunostimulants) to test these as an antiparasitic strategy in the newly established in vivo system.

Název v anglickém jazyce

Testing parasiticidal and immunomodulatory substances to combat myxozoan infections in aquaculture using novel in vitro and in vivo models

Popis výsledku anglicky

In the initiation phase of the project we reared and/or acquired approx. 3000 specific pathogen-free carp for experimental use. We used these fish to perform repeated intraperitoneal infection challenges with S. molnari blood stages and to monitor parasite proliferation over time using qPCR. Based on maximum parasite numbers in the blood, we have now established a continuous in vivo production line for parasite blood stages every 28 days (G2 – technically realized result/technicky realizované výsledky – prototyp, funkční vzorek) which allows us to be independent from the collection of environmental samples which is especially hindered in the winter months (frozen ponds, low temperature and undetectable parasite loads). Blood stages are harvested by centrifugation and used for in vivo and in vitro trials. We have performed a hematological study on carp blood and developed an immunoglobulin M sec qPCR assay to determine the immunological response of carp to parasite infection (in preparation for publication). Furthermore, together with Skretting ARC and Lallemond Animal Nutrition we have composed recipes for two carp diets, one based on curcumin (parasiticidal substance) and one based on yeast and probiotics (immunostimulants) to test these as an antiparasitic strategy in the newly established in vivo system.

Druh

G_funk - Funkční vzorek

CEP obor

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OECD FORD obor

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Projekt

<a href="/cs/project/TG02010034" target="_blank" >TG02010034: BCAV21 - Podpora komercializace výsledků VaV na BC</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Interní identifikační kód produktu

TG02010034_Rybomorky_Holzer

Číselná identifikace

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Technické parametry

The presented functional sample represents a continuous in vivo culture and transmission system for blood stages of the myxozoan parasite S. molnari. S. molnari blood stages (SMBS) are transmitted to specific pathogen-free (SPF) fish via intraperitoneal injection and are harvested from host blood after 28 days, when parasite levels are at their peak. SMBS are then collected and used to infect a new lot of SPF carp. The aim of this process is 1. To be able to produce large numbers of SMBS throughout the year, independent from environmental conditions (frozen ponds during the winter months), 2. To work in a ‘clean’ culture system devoid of co-infections with other parasites, fungi or bacteria, usually present in ponds, and 3. To be able to test the effect of potentially parasiticidal or immunomodulatory substances in an in vivo model, aiming at essential reduction/removal of myxozoan proliferative stages. If effective, such substances could then be included into anti- parasitic diets for fish, with S. molnari serving as a model for other myxozoan infections in fish in aquaculture systems worldwide. This is of particular importance as a treatment against myxozoans in fish destined for human consumption is presently unavailable.

Ekonomické parametry

Předpokládaným výstupem je prodej licence know-how a z toho plynoucí licenční poplatky pro výzkumnou instituci. Odhad zisku pro výzkumnou instituci je 300 000,- Kč.

Kategorie aplik. výsledku dle nákladů

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IČO vlastníka výsledku

60077344

Název vlastníka

Biologické centrum

Stát vlastníka

CZ - Česká republika

Druh možnosti využití

P - Využití výsledku jiným subjektem je v některých případech možné bez nabytí licence

Požadavek na licenční poplatek

A - Poskytovatel licence na výsledek požaduje licenční poplatek

Adresa www stránky s výsledkem

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