Mitochondrion remodeling during T. b. brucei developmental differentiation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00488337" target="_blank" >RIV/60077344:_____/17:00488337 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60077344:_____/17:00488338
Výsledek na webu
—
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Mitochondrion remodeling during T. b. brucei developmental differentiation
Popis výsledku v původním jazyce
Trypanosoma brucei undergoes a complex life cycle as it alternates between a mammalian host and the blood-feeding insect vector, a tsetse fly. Due to the different environments, the distinct life stages differ in their energy metabolism, i.e. insect stage (procyclic cells, PS) depends on mitochondrial oxidative phosphorylation (OXPHOS) for ATP production while the bloodstream stage (BS) gains energy by aerobic glycolysis. The dramatic switch from the OXPHOS to glycolysis happens during the complex development of the PS in the tsetse fly. This development differentiation is characterized by extensive remodeling of mitochondrion structure and changes in mitochondrial bioenergetics. Importantly, the molecular mechanism behind this process is completely unknown. We have established the in vitro differentiation system, in which the transition from PS to epimastigotes followed by differentiation to transmission-ready metacylic trypanosomes is triggered by RNA binding protein 6 (RBP6) expression. This in vitro induced differentiation of PF cells takes 8 days. The appearance of epimastigotes and metacyclic trypanosomes in the culture was mapped using light and fluorescent microscopy. The whole cell proteome of cell culture harvested every day after the RBP6 induction was identified by label-free quantitative mass spectrometry. This proteomic data serves as a resource for further detailed characterization of changes happening in the parasite mitochondrion as well as identification of possible candidates involved in the PS differentiation.
Název v anglickém jazyce
Mitochondrion remodeling during T. b. brucei developmental differentiation
Popis výsledku anglicky
Trypanosoma brucei undergoes a complex life cycle as it alternates between a mammalian host and the blood-feeding insect vector, a tsetse fly. Due to the different environments, the distinct life stages differ in their energy metabolism, i.e. insect stage (procyclic cells, PS) depends on mitochondrial oxidative phosphorylation (OXPHOS) for ATP production while the bloodstream stage (BS) gains energy by aerobic glycolysis. The dramatic switch from the OXPHOS to glycolysis happens during the complex development of the PS in the tsetse fly. This development differentiation is characterized by extensive remodeling of mitochondrion structure and changes in mitochondrial bioenergetics. Importantly, the molecular mechanism behind this process is completely unknown. We have established the in vitro differentiation system, in which the transition from PS to epimastigotes followed by differentiation to transmission-ready metacylic trypanosomes is triggered by RNA binding protein 6 (RBP6) expression. This in vitro induced differentiation of PF cells takes 8 days. The appearance of epimastigotes and metacyclic trypanosomes in the culture was mapped using light and fluorescent microscopy. The whole cell proteome of cell culture harvested every day after the RBP6 induction was identified by label-free quantitative mass spectrometry. This proteomic data serves as a resource for further detailed characterization of changes happening in the parasite mitochondrion as well as identification of possible candidates involved in the PS differentiation.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/LL1205" target="_blank" >LL1205: Charakterizace unikátních vlastností esenciální FoF1 ATP syntázy u původce africké spavé nemoci Trypanosoma bucei za účelem vývoje inhibitorů tohoto komplexu.</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů