GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F18%3A00493749" target="_blank" >RIV/60077344:_____/18:00493749 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-7643-0_11" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7643-0_11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-7643-0_11" target="_blank" >10.1007/978-1-4939-7643-0_11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol

Popis výsledku v původním jazyce

A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.

Název v anglickém jazyce

GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol

Popis výsledku anglicky

A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/GA17-22276S" target="_blank" >GA17-22276S: Nové metody pro metabolomickou analýzu obtížně stanovitelných metabolitů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Metabolomic Profiling: Methods and Protocols, Methods in Molecular Biology

ISBN

978-1-4939-7642-3

Počet stran výsledku

23

Strana od-do

159-181

Počet stran knihy

291

Název nakladatele

Humana Press

Místo vydání

New York

Kód UT WoS kapitoly

000441296900012