Protura are unique: First evidence of specialized feeding on ectomycorrhizal fungi in soil invertebrates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00505441" target="_blank" >RIV/60077344:_____/19:00505441 - isvavai.cz</a>

Výsledek na webu

<a href="https://bmcecol.biomedcentral.com/track/pdf/10.1186/s12898-019-0227-y" target="_blank" >https://bmcecol.biomedcentral.com/track/pdf/10.1186/s12898-019-0227-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12898-019-0227-y" target="_blank" >10.1186/s12898-019-0227-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protura are unique: First evidence of specialized feeding on ectomycorrhizal fungi in soil invertebrates

Popis výsledku v původním jazyce

Background: Ectomycorrhizal fungi (ECM) play a central role in nutrient cycling in boreal and temperate forests, but their role in the soil food web remains little understood. One of the groups assumed to live as specialised mycorrhizal feeders are Protura, but experimental and field evidence is lacking. We used a combination of three methods to test if Protura are specialized mycorrhizal feeders and compared their trophic niche with other soil invertebrates. Using pulse labelling of young beech and ash seedlings we analysed the incorporation of 13 C and 15 N into Acerentomon gallicum. In addition, individuals of Protura from temperate forests were collected for the analysis of neutral lipid fatty acids and natural variations in stable isotope ratios. nResults: Pulse labelling showed rapid incorporation of root-derived 13 C, but no incorporation of root-derived 15 N into A. gallicum. The transfer of 13 C from lateral roots to ectomycorrhizal root tips was high, while it was low for 15 N. Neutral lipid fatty acid (NLFA) analysis showed high amounts of bacterial marker (16:1ω7) and plant marker (16:0 and 18:1ω9) fatty acids but not of the fungal membrane lipid 18:2ω6,9 in A. gallicum. Natural variations in stable isotope ratios in Protura from a number of temperate forests were distinct from those of the great majority of other soil invertebrates, but remarkably similar to those of sporocarps of ECM fungi. nConclusions: Using three in situ methods, stable isotope labelling, neutral lipid fatty acid analysis and natural variations of stable isotope ratios, we showed that Protura predominantly feed on mycorrhizal hyphae via sucking up hyphal cytoplasm. Predominant feeding on ectomycorrhizal mycelia by Protura is an exception, the limited consumption of ECM by other soil invertebrates may contribute to carbon sequestration in temperate and boreal forests.

Název v anglickém jazyce

Protura are unique: First evidence of specialized feeding on ectomycorrhizal fungi in soil invertebrates

Popis výsledku anglicky

Background: Ectomycorrhizal fungi (ECM) play a central role in nutrient cycling in boreal and temperate forests, but their role in the soil food web remains little understood. One of the groups assumed to live as specialised mycorrhizal feeders are Protura, but experimental and field evidence is lacking. We used a combination of three methods to test if Protura are specialized mycorrhizal feeders and compared their trophic niche with other soil invertebrates. Using pulse labelling of young beech and ash seedlings we analysed the incorporation of 13 C and 15 N into Acerentomon gallicum. In addition, individuals of Protura from temperate forests were collected for the analysis of neutral lipid fatty acids and natural variations in stable isotope ratios. nResults: Pulse labelling showed rapid incorporation of root-derived 13 C, but no incorporation of root-derived 15 N into A. gallicum. The transfer of 13 C from lateral roots to ectomycorrhizal root tips was high, while it was low for 15 N. Neutral lipid fatty acid (NLFA) analysis showed high amounts of bacterial marker (16:1ω7) and plant marker (16:0 and 18:1ω9) fatty acids but not of the fungal membrane lipid 18:2ω6,9 in A. gallicum. Natural variations in stable isotope ratios in Protura from a number of temperate forests were distinct from those of the great majority of other soil invertebrates, but remarkably similar to those of sporocarps of ECM fungi. nConclusions: Using three in situ methods, stable isotope labelling, neutral lipid fatty acid analysis and natural variations of stable isotope ratios, we showed that Protura predominantly feed on mycorrhizal hyphae via sucking up hyphal cytoplasm. Predominant feeding on ectomycorrhizal mycelia by Protura is an exception, the limited consumption of ECM by other soil invertebrates may contribute to carbon sequestration in temperate and boreal forests.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Ecology

ISSN

1472-6785

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

10

Kód UT WoS článku

000459477900001

EID výsledku v databázi Scopus

2-s2.0-85062029709