Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00511209" target="_blank" >RIV/60077344:_____/19:00511209 - isvavai.cz</a>

Výsledek na webu

<a href="https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13065" target="_blank" >https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13065</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppa.13065" target="_blank" >10.1111/ppa.13065</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Popis výsledku v původním jazyce

Three pairs of generic primers designed for specific reverse transcription polymerase chain reaction amplification of part of the cytorhabdoviruses genomes were successfully tested with a number of red clover samples. Two infected plants showing irregular vein clearing, tissue necrosis and stunted growth symptoms were selected for further studies. Subsequently, two novel plant rhabdoviruses, tentatively named Trifolium pratense virus A (TpVA) and Trifolium pratense virus B (TpVB), were detected in red clover plants. Sequence analyses of nucleotide sequences of their genomic RNAs obtained after high-throughput sequencing confirmed their affinity to members of the genus Cytorhabdovirus. While TpVA was transmitted by mechanical inoculation to Nicotiana occidentalis 37B and subtransferred to Physalis floridana plants, crude sap transmission of TpVB to a range of herbaceous host plants failed. The bacilliform shape of virions, which has a cytoplasm-limited distribution, their sizes, and the phylogenetic relationships, support assignment of TpVA and TpVB to two distinct species of the genus Cytorhabdovirus.

Název v anglickém jazyce

Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Popis výsledku anglicky

Three pairs of generic primers designed for specific reverse transcription polymerase chain reaction amplification of part of the cytorhabdoviruses genomes were successfully tested with a number of red clover samples. Two infected plants showing irregular vein clearing, tissue necrosis and stunted growth symptoms were selected for further studies. Subsequently, two novel plant rhabdoviruses, tentatively named Trifolium pratense virus A (TpVA) and Trifolium pratense virus B (TpVB), were detected in red clover plants. Sequence analyses of nucleotide sequences of their genomic RNAs obtained after high-throughput sequencing confirmed their affinity to members of the genus Cytorhabdovirus. While TpVA was transmitted by mechanical inoculation to Nicotiana occidentalis 37B and subtransferred to Physalis floridana plants, crude sap transmission of TpVB to a range of herbaceous host plants failed. The bacilliform shape of virions, which has a cytoplasm-limited distribution, their sizes, and the phylogenetic relationships, support assignment of TpVA and TpVB to two distinct species of the genus Cytorhabdovirus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Pathology

ISSN

0032-0862

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

1276-1286

Kód UT WoS článku

000478739800005

EID výsledku v databázi Scopus

2-s2.0-85069930627