Shifts in cell size and community composition of bacterioplankton due to grazing by heterotrophic flagellates: evidence from a marine system

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00517802" target="_blank" >RIV/60077344:_____/19:00517802 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.int-res.com/abstracts/ame/v83/n3/p295-308/" target="_blank" >https://www.int-res.com/abstracts/ame/v83/n3/p295-308/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3354/ame01919" target="_blank" >10.3354/ame01919</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Shifts in cell size and community composition of bacterioplankton due to grazing by heterotrophic flagellates: evidence from a marine system

Popis výsledku v původním jazyce

The effect of flagellate grazing on bacterioplankton community composition, e.g. cell size and diversity, has been well studied in fresh waters, but much less is known for marine systems. We conducted experiments with communities from an oligotrophic bay in the NW Mediterranean Sea, size fractionation was used to alter grazing by flagellates, and incubation in dialysis bags was used to retain otherwise in situ conditions. Grazing reduced bacterial abundance and growth. In contrast to a current model of the effects of grazing, cell size distribution was unimodal and not bimodal. Significant shifts towards larger cell size classes were observed. This is in accordance with the idea that increasing the cell length is a bacterial strategy to reduce susceptibility to grazing by small flagellates. Only weak evidence was found for the complementary strategy of reducing cell size. In all experiments, bacterial community structure as assessed by PCR-based 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) showed significant differences associated with the presence of grazers. Sequence analyses of DGGE bands were used to select probes for catalyzed reporter deposition-fluorescence in situ hybridization analysis for specific taxa. In our experiments, Polaribacter showed patterns of defense against grazing, while Roseobacter was highly susceptible to grazing. Although the effect of grazing may be stronger in freshwater than in oligotrophic marine systems, our data support the hypotheses that (1) increasing the cell size is a bacterial defense strategy against grazing by small flagellates typical in marine systems and (2) grazing influences bacterial community composition.n

Název v anglickém jazyce

Shifts in cell size and community composition of bacterioplankton due to grazing by heterotrophic flagellates: evidence from a marine system

Popis výsledku anglicky

The effect of flagellate grazing on bacterioplankton community composition, e.g. cell size and diversity, has been well studied in fresh waters, but much less is known for marine systems. We conducted experiments with communities from an oligotrophic bay in the NW Mediterranean Sea, size fractionation was used to alter grazing by flagellates, and incubation in dialysis bags was used to retain otherwise in situ conditions. Grazing reduced bacterial abundance and growth. In contrast to a current model of the effects of grazing, cell size distribution was unimodal and not bimodal. Significant shifts towards larger cell size classes were observed. This is in accordance with the idea that increasing the cell length is a bacterial strategy to reduce susceptibility to grazing by small flagellates. Only weak evidence was found for the complementary strategy of reducing cell size. In all experiments, bacterial community structure as assessed by PCR-based 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) showed significant differences associated with the presence of grazers. Sequence analyses of DGGE bands were used to select probes for catalyzed reporter deposition-fluorescence in situ hybridization analysis for specific taxa. In our experiments, Polaribacter showed patterns of defense against grazing, while Roseobacter was highly susceptible to grazing. Although the effect of grazing may be stronger in freshwater than in oligotrophic marine systems, our data support the hypotheses that (1) increasing the cell size is a bacterial defense strategy against grazing by small flagellates typical in marine systems and (2) grazing influences bacterial community composition.n

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA13-00243S" target="_blank" >GA13-00243S: Charakteristiky životních strategií vybraných skupin Betaproteobacteria ve vztahu k jejich roli v přenosu uhlíku do vyšších trofických hladin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquatic Microbial Ecology

ISSN

0948-3055

e-ISSN

—

Svazek periodika

83

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

295-308

Kód UT WoS článku

000522814600008

EID výsledku v databázi Scopus

2-s2.0-85076235123