Intranuclear coccidiosis in tortoises discovery of its causative agent and transmission

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00519673" target="_blank" >RIV/60077344:_____/19:00519673 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/19:43899174 RIV/62157124:16170/19:43876318

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S093247391830083X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S093247391830083X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejop.2018.11.002" target="_blank" >10.1016/j.ejop.2018.11.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Intranuclear coccidiosis in tortoises discovery of its causative agent and transmission

Popis výsledku v původním jazyce

Intranuclear coccidiosis of itestudines (known as TINC) is an emerging disease in chelonians. Although endogenous stages were repeatedly detected in various tissues, attempts to find the oocysts in faeces failed, leaving the question of the transmission and classification of the causative agent of TINC unresolved. We recorded small spherical oocysts (similar to 6-7 mu m in diameter) of an eimeriid coccidium in faeces of a leopard tortoise (Stigmochelys pardalis). Sporulated oocysts were used for the experimental oral inoculation of juvenile coccidia-free tortoises representing 5 species (S. pardalis, Testudo graeca, T. hermanni, T. horsfieldii, and Geochelone sulcata). The oocysts' association with TINC was confirmed based on clinical signs, histopathological findings of intranuclear endogenous stages of the coccidium in many organs (including intestine), and by the partial 18S rDNA sequence analysis of the DNA isolated from organs of the experimentally infected animals and from a single naturally infected as well as from all experimentally infected tortoises. Breeding colonies of chelonians should be screened for this pathogen in order to prevent its further spread and unwanted introduction into endangered free-ranging chelonian populations. (C) 2018 Elsevier GmbH. All rights reserved.

Název v anglickém jazyce

Intranuclear coccidiosis in tortoises discovery of its causative agent and transmission

Popis výsledku anglicky

Intranuclear coccidiosis of itestudines (known as TINC) is an emerging disease in chelonians. Although endogenous stages were repeatedly detected in various tissues, attempts to find the oocysts in faeces failed, leaving the question of the transmission and classification of the causative agent of TINC unresolved. We recorded small spherical oocysts (similar to 6-7 mu m in diameter) of an eimeriid coccidium in faeces of a leopard tortoise (Stigmochelys pardalis). Sporulated oocysts were used for the experimental oral inoculation of juvenile coccidia-free tortoises representing 5 species (S. pardalis, Testudo graeca, T. hermanni, T. horsfieldii, and Geochelone sulcata). The oocysts' association with TINC was confirmed based on clinical signs, histopathological findings of intranuclear endogenous stages of the coccidium in many organs (including intestine), and by the partial 18S rDNA sequence analysis of the DNA isolated from organs of the experimentally infected animals and from a single naturally infected as well as from all experimentally infected tortoises. Breeding colonies of chelonians should be screened for this pathogen in order to prevent its further spread and unwanted introduction into endangered free-ranging chelonian populations. (C) 2018 Elsevier GmbH. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Protistology

ISSN

0932-4739

e-ISSN

—

Svazek periodika

67

Číslo periodika v rámci svazku

FEB 2019

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

71-76

Kód UT WoS článku

000460396700006

EID výsledku v databázi Scopus

2-s2.0-85057118279