Utilization of dietary protein in the litter-dwelling larva of Bibio marci (Diptera: Bibionidae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00520728" target="_blank" >RIV/60077344:_____/19:00520728 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1134%2FS1064229319120032" target="_blank" >https://link.springer.com/article/10.1134%2FS1064229319120032</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1134/S1064229319120032" target="_blank" >10.1134/S1064229319120032</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of dietary protein in the litter-dwelling larva of Bibio marci (Diptera: Bibionidae)

Popis výsledku v původním jazyce

As plant litter is typically N limited, protein digestion and amino acid assimilation are key processes in saprophagous invertebrates. The recalcitrance of dietary protein may be increased by their binding to phenolic compounds in humic substances. Binding phenolic compound to protein may be also important in humus acid formation and stabilization of nitrogen bounded protein in soil organic matter. Consequently, better understanding of interaction of protein and phenolic compounds in invertebrate gut is important not only from ecophysiology perspective but also in terms of soil organic matter dynamics. In this study, we evaluated the digestive efficiency of proteins during gut passage in the litter-dwelling larva of Bibio marci. Anterior part of the midgut is highly alkaline, which agrees with the protease pH optimum. We found large differences in protease activities of the leaf litter, gut, and excrements. However, individual gut compartments were close to one another in their protease activities. The same was true for the contents of amino acids and ammonia. Also, amino acid composition differed between compartments. Litter had the high content of glutamine and serine, whereas hindgut and excrement were characterized by the high content of methionine, and the remaining gut segments had the high content of valine. Experiment with 14C-labelled protein demonstrated that gut passage improved the digestive efficiency of proteins, especially for a humic acid stabilized protein.

Název v anglickém jazyce

Utilization of dietary protein in the litter-dwelling larva of Bibio marci (Diptera: Bibionidae)

Popis výsledku anglicky

As plant litter is typically N limited, protein digestion and amino acid assimilation are key processes in saprophagous invertebrates. The recalcitrance of dietary protein may be increased by their binding to phenolic compounds in humic substances. Binding phenolic compound to protein may be also important in humus acid formation and stabilization of nitrogen bounded protein in soil organic matter. Consequently, better understanding of interaction of protein and phenolic compounds in invertebrate gut is important not only from ecophysiology perspective but also in terms of soil organic matter dynamics. In this study, we evaluated the digestive efficiency of proteins during gut passage in the litter-dwelling larva of Bibio marci. Anterior part of the midgut is highly alkaline, which agrees with the protease pH optimum. We found large differences in protease activities of the leaf litter, gut, and excrements. However, individual gut compartments were close to one another in their protease activities. The same was true for the contents of amino acids and ammonia. Also, amino acid composition differed between compartments. Litter had the high content of glutamine and serine, whereas hindgut and excrement were characterized by the high content of methionine, and the remaining gut segments had the high content of valine. Experiment with 14C-labelled protein demonstrated that gut passage improved the digestive efficiency of proteins, especially for a humic acid stabilized protein.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/GA17-14409S" target="_blank" >GA17-14409S: Úloha vlastností druhů půdní fauny v jejich vlivu na dekomposici a stabilizaci půdní organické hmoty</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Eurasian Soil Science

ISSN

1064-2293

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

5

Strana od-do

1583-1587

Kód UT WoS článku

000510611800010

EID výsledku v databázi Scopus

2-s2.0-85075443057