Complex evolution of insect insulin receptors and homologous decoy receptors, and functional significance of their multiplicity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00523500" target="_blank" >RIV/60077344:_____/20:00523500 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/20:00539924 RIV/61388963:_____/20:00523500 RIV/60076658:12310/20:43901227

Výsledek na webu

<a href="https://academic.oup.com/mbe/article-pdf/doi/10.1093/molbev/msaa048/32984781/msaa048.pdf" target="_blank" >https://academic.oup.com/mbe/article-pdf/doi/10.1093/molbev/msaa048/32984781/msaa048.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/molbev/msaa048" target="_blank" >10.1093/molbev/msaa048</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Complex evolution of insect insulin receptors and homologous decoy receptors, and functional significance of their multiplicity

Popis výsledku v původním jazyce

Evidence accumulates that the functional plasticity of insulin and insulin-like growth factor signaling (IIS) in insects could spring, among others, from the multiplicity of insulin receptors (InRs). Their multiple variants may be implemented in the control of insect polyphenism, such as wing or caste polyphenism. Here, we present a comprehensive phylogenetic analysis of insect InR sequences in 118 species from 23 orders and investigate the role of three InRs identified in the linden bug, Pyrrhocoris apterus, in wing polymorphism control. We identified two gene clusters (Cluster I and II) resulting from an ancestral duplication in a late ancestor of winged insects, which remained conserved in most lineages, only in some of them being subject to further duplications or losses. One remarkable yet neglected feature of InR evolution is the loss of the tyrosine kinase catalytic domain, giving rise to decoys of insulin receptor in both clusters. Within the Cluster I, we confirmed the presence of the secreted decoy of insulin receptor (SDR) in all studied Muscomorpha. More importantly, we described a new tyrosine kinase-less gene (DR2) in the Cluster II, conserved in apical Holometabola for 300 million years. We differentially silenced the three P. apterus InRs and confirmed their participation in wing polymorphism control. We observed a pattern of Cluster I and II InRs impact on wing development, which differed from that postulated in planthoppers, suggesting an independent establishment of IIS control over wing development, leading to idiosyncrasies in the cooption of multiple InRs in polyphenism control in different taxa.

Název v anglickém jazyce

Complex evolution of insect insulin receptors and homologous decoy receptors, and functional significance of their multiplicity

Popis výsledku anglicky

Evidence accumulates that the functional plasticity of insulin and insulin-like growth factor signaling (IIS) in insects could spring, among others, from the multiplicity of insulin receptors (InRs). Their multiple variants may be implemented in the control of insect polyphenism, such as wing or caste polyphenism. Here, we present a comprehensive phylogenetic analysis of insect InR sequences in 118 species from 23 orders and investigate the role of three InRs identified in the linden bug, Pyrrhocoris apterus, in wing polymorphism control. We identified two gene clusters (Cluster I and II) resulting from an ancestral duplication in a late ancestor of winged insects, which remained conserved in most lineages, only in some of them being subject to further duplications or losses. One remarkable yet neglected feature of InR evolution is the loss of the tyrosine kinase catalytic domain, giving rise to decoys of insulin receptor in both clusters. Within the Cluster I, we confirmed the presence of the secreted decoy of insulin receptor (SDR) in all studied Muscomorpha. More importantly, we described a new tyrosine kinase-less gene (DR2) in the Cluster II, conserved in apical Holometabola for 300 million years. We differentially silenced the three P. apterus InRs and confirmed their participation in wing polymorphism control. We observed a pattern of Cluster I and II InRs impact on wing development, which differed from that postulated in planthoppers, suggesting an independent establishment of IIS control over wing development, leading to idiosyncrasies in the cooption of multiple InRs in polyphenism control in different taxa.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Biology and Evolution

ISSN

0737-4038

e-ISSN

—

Svazek periodika

37

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

1775-1789

Kód UT WoS článku

000569060900018

EID výsledku v databázi Scopus

2-s2.0-85084206628