Long-term effects of earthworms (Lumbricus rubellus Hoffmeister, 1843) on activity and composition of soil microbial community under laboratory conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00524387" target="_blank" >RIV/60077344:_____/20:00524387 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/20:00524387 RIV/00216208:11310/20:10414083

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S092913931930873X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S092913931930873X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apsoil.2019.103463" target="_blank" >10.1016/j.apsoil.2019.103463</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Long-term effects of earthworms (Lumbricus rubellus Hoffmeister, 1843) on activity and composition of soil microbial community under laboratory conditions

Popis výsledku v původním jazyce

Observations of earthworm colonization on sites where they were absent previously, such as on post-mining heaps, indicate that they may substantially alter soil conditions as well as soil microbial communities. However, long-term effects of earthworms on microbial activity of soil ecosystems remains poorly understood. To improve this situation, we set up microcosm experiments with clay only, with clay and litter, and with clay, litter and earthworms. Microcosms were sampled after 3, 60, 180 and 395 days of cultivation and divided into three subsamples: clay, litter, and drilosphere (earthworm tunnels and associates casts), when present. Microbial respiration, microbial biomass carbon, species composition, metabolic activity using BIOLOG, PLFA concentration, isoprenoid quinones content and DGGE fingerprints were analysed in all microcosms and at all subsamples. Microbial biomass carbon and basal soil respiration were significantly affected by type of microcosms and time of sampling. Microbial biomass in drilosphere was significantly higher than in clay and litter. The CFU (colony forming units) were significantly affected by presence of earthworms having significantly higher CFU in drilosphere than in all other subsamples. Analysis of PLFA indicated highest relative biomass of fungi and bacteria in clay from microcosms with earthworms. The DGGE analysis showed various compositions of microbial communities among subsamples from various treatments taken at different time. Finally, our results revealed significant effect of earthworms on activity and biomass of soil microbial community under long-term laboratory incubation.

Název v anglickém jazyce

Long-term effects of earthworms (Lumbricus rubellus Hoffmeister, 1843) on activity and composition of soil microbial community under laboratory conditions

Popis výsledku anglicky

Observations of earthworm colonization on sites where they were absent previously, such as on post-mining heaps, indicate that they may substantially alter soil conditions as well as soil microbial communities. However, long-term effects of earthworms on microbial activity of soil ecosystems remains poorly understood. To improve this situation, we set up microcosm experiments with clay only, with clay and litter, and with clay, litter and earthworms. Microcosms were sampled after 3, 60, 180 and 395 days of cultivation and divided into three subsamples: clay, litter, and drilosphere (earthworm tunnels and associates casts), when present. Microbial respiration, microbial biomass carbon, species composition, metabolic activity using BIOLOG, PLFA concentration, isoprenoid quinones content and DGGE fingerprints were analysed in all microcosms and at all subsamples. Microbial biomass carbon and basal soil respiration were significantly affected by type of microcosms and time of sampling. Microbial biomass in drilosphere was significantly higher than in clay and litter. The CFU (colony forming units) were significantly affected by presence of earthworms having significantly higher CFU in drilosphere than in all other subsamples. Analysis of PLFA indicated highest relative biomass of fungi and bacteria in clay from microcosms with earthworms. The DGGE analysis showed various compositions of microbial communities among subsamples from various treatments taken at different time. Finally, our results revealed significant effect of earthworms on activity and biomass of soil microbial community under long-term laboratory incubation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40104 - Soil science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Soil Ecology

ISSN

0929-1393

e-ISSN

—

Svazek periodika

150

Číslo periodika v rámci svazku

JUN 2020

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

103463

Kód UT WoS článku

000512886800018

EID výsledku v databázi Scopus

2-s2.0-85076486246