Direct inhibition of photosynthesis by Cd dominates over inhibition caused by micronutrient deficiency in the Cd/Zn hyperaccumulator Arabidopsis halleri.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00532421" target="_blank" >RIV/60077344:_____/20:00532421 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0981942820303521?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0981942820303521?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2020.07.018" target="_blank" >10.1016/j.plaphy.2020.07.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direct inhibition of photosynthesis by Cd dominates over inhibition caused by micronutrient deficiency in the Cd/Zn hyperaccumulator Arabidopsis halleri.

Popis výsledku v původním jazyce

This work reveals, by imaging in vivo measurements in the Cd/Zn hyperaccumulator Arabidopsis halleri, in how far Cd stress affects macronutrient (Ca, K) and micronutrient (Fe, Zn) distribution in the leaves. We directly correlate these changes with the biophysics of the photosynthetic light reactions. Plants were grown for 2monthsat 10muM Zn (=control) and supplemented with 10, 15, 50, or 75muM Cd. Direct imaging of OJIP transients revealed that bundle sheath cells were more sensitive to Cd toxicity than mesophyll cells further from the vein. Progressive inhibition of photosystem (PS) II reaction centers and a decrease in quantum yield of electron transport between QA and QB and further to PSI acceptors was observed. This was correlated with the decreased dynamics of QA re-oxidation and lower operating efficiency of PSII. Analysis by a benchtop micro X-ray fluorescence device showed that Cd mostly accumulated in the veins, and restricted Fe and Zn distribution from the veins, especially in the 75muM Cd, while K concentration increased in the whole leaf. Calcium distribution was apparently not affected by Cd, but Cd excess inhibited trichome formation and thereby diminished total Ca concentration in the leaves. The results point to differential tissue sensitivity to Cd, evident by heterogeneous inhibition of photosynthesis. Part of this may be a result of selective disturbances in the leaf nutrient homeostasis. The better photosynthetic performance away from the veins compared to the bundle sheath cells, however, indicates that direct inhibition of photosynthesis by Cd dominates over inhibition caused by micronutrient deficiency.

Název v anglickém jazyce

Direct inhibition of photosynthesis by Cd dominates over inhibition caused by micronutrient deficiency in the Cd/Zn hyperaccumulator Arabidopsis halleri.

Popis výsledku anglicky

This work reveals, by imaging in vivo measurements in the Cd/Zn hyperaccumulator Arabidopsis halleri, in how far Cd stress affects macronutrient (Ca, K) and micronutrient (Fe, Zn) distribution in the leaves. We directly correlate these changes with the biophysics of the photosynthetic light reactions. Plants were grown for 2monthsat 10muM Zn (=control) and supplemented with 10, 15, 50, or 75muM Cd. Direct imaging of OJIP transients revealed that bundle sheath cells were more sensitive to Cd toxicity than mesophyll cells further from the vein. Progressive inhibition of photosystem (PS) II reaction centers and a decrease in quantum yield of electron transport between QA and QB and further to PSI acceptors was observed. This was correlated with the decreased dynamics of QA re-oxidation and lower operating efficiency of PSII. Analysis by a benchtop micro X-ray fluorescence device showed that Cd mostly accumulated in the veins, and restricted Fe and Zn distribution from the veins, especially in the 75muM Cd, while K concentration increased in the whole leaf. Calcium distribution was apparently not affected by Cd, but Cd excess inhibited trichome formation and thereby diminished total Ca concentration in the leaves. The results point to differential tissue sensitivity to Cd, evident by heterogeneous inhibition of photosynthesis. Part of this may be a result of selective disturbances in the leaf nutrient homeostasis. The better photosynthetic performance away from the veins compared to the bundle sheath cells, however, indicates that direct inhibition of photosynthesis by Cd dominates over inhibition caused by micronutrient deficiency.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/EF15_003%2F0000336" target="_blank" >EF15_003/0000336: Kovy, rostliny a lidé</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

—

Svazek periodika

155

Číslo periodika v rámci svazku

Oct 2020

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

10

Strana od-do

252-261

Kód UT WoS článku

000581918000024

EID výsledku v databázi Scopus

2-s2.0-85089498789