Fragaria vesca-associated virus 1: a new virus related to negeviruses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00532668" target="_blank" >RIV/60077344:_____/20:00532668 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs00705-020-04603-9" target="_blank" >https://link.springer.com/article/10.1007%2Fs00705-020-04603-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00705-020-04603-9" target="_blank" >10.1007/s00705-020-04603-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fragaria vesca-associated virus 1: a new virus related to negeviruses

Popis výsledku v původním jazyce

In this study, the complete genomic sequence of a novel virus was determined by next-generation sequencing of a sample from a symptomatic strawberry plant with severe yellow spots and mosaic on its leaves. Its genomic organization and sequence showed that this virus is related to members of the proposed insect-specific genus Negevirus. The sample also contained sequences from the geranium aphid Acyrthosiphon malvae. Although the virus was detected repeatedly in the same plant during the three following years, no other positive samples were obtained from the surroundings or more-distant locations. Reverse transcription qPCR analysis revealed the presence of both genomic positive and complementary negative strands of the viral genome in the sample, with a 3- to 30-fold excess of the positive strand, indicating active viral replication. As the virus was not detected in any insect species collected at this location, the virus was provisionally named Fragaria vesca-associated virus 1 (FVaV-1).

Název v anglickém jazyce

Fragaria vesca-associated virus 1: a new virus related to negeviruses

Popis výsledku anglicky

In this study, the complete genomic sequence of a novel virus was determined by next-generation sequencing of a sample from a symptomatic strawberry plant with severe yellow spots and mosaic on its leaves. Its genomic organization and sequence showed that this virus is related to members of the proposed insect-specific genus Negevirus. The sample also contained sequences from the geranium aphid Acyrthosiphon malvae. Although the virus was detected repeatedly in the same plant during the three following years, no other positive samples were obtained from the surroundings or more-distant locations. Reverse transcription qPCR analysis revealed the presence of both genomic positive and complementary negative strands of the viral genome in the sample, with a 3- to 30-fold excess of the positive strand, indicating active viral replication. As the virus was not detected in any insect species collected at this location, the virus was provisionally named Fragaria vesca-associated virus 1 (FVaV-1).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of Virology

ISSN

0304-8608

e-ISSN

—

Svazek periodika

165

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

4

Strana od-do

1249-1252

Kód UT WoS článku

000522863300003

EID výsledku v databázi Scopus

2-s2.0-85082760765