Characterization of sponge-associated Verrucomicrobia: microcompartment-based sugar utilization and enhanced toxin-antitoxin modules as features of host-associated Opitutales

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00538548" target="_blank" >RIV/60077344:_____/20:00538548 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1111/1462-2920.15210" target="_blank" >https://doi.org/10.1111/1462-2920.15210</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1462-2920.15210" target="_blank" >10.1111/1462-2920.15210</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of sponge-associated Verrucomicrobia: microcompartment-based sugar utilization and enhanced toxin-antitoxin modules as features of host-associated Opitutales

Popis výsledku v původním jazyce

Bacteria of the phylumVerrucomicrobiaare ubiquitous in marine environments and can be found as free-living organisms or as symbionts of eukaryotic hosts. Little is known about host-associatedVerrucomicrobiain the marine environment. Here we reconstructed two genomes of symbioticVerrucomicrobiafrom bacterial metagenomes derived from the Atlanto-Mediterranean spongePetrosia ficiformisand three genomes from strains that we isolated from offshore seawater of the Eastern Mediterranean Sea. Phylogenomic analysis of these five strains indicated that they are all members ofVerrucomicrobiasubdivision 4, orderOpitutales. We compared these novel sponge-associated and seawater-isolated genomes to closely relatedVerrucomicrobia. Genomic analysis revealed thatPlanctomycetes-Verrucomicrobiamicrocompartment gene clusters are enriched in the genomes of symbioticOpitutalesincluding sponge symbionts but not in free-living ones. We hypothesize that in sponge symbionts these microcompartments are used for degradation ofl-fucose andl-rhamnose, which are components of algal and bacterial cell walls and therefore may be found at high concentrations in the sponge tissue. Furthermore, we observed an enrichment of toxin-antitoxin modules in symbioticOpitutales. We suggest that, in sponges, verrucomicrobial symbionts utilize these modules as a defence mechanism against antimicrobial activity deriving from the abundant microbial community co-inhabiting the host.

Název v anglickém jazyce

Characterization of sponge-associated Verrucomicrobia: microcompartment-based sugar utilization and enhanced toxin-antitoxin modules as features of host-associated Opitutales

Popis výsledku anglicky

Bacteria of the phylumVerrucomicrobiaare ubiquitous in marine environments and can be found as free-living organisms or as symbionts of eukaryotic hosts. Little is known about host-associatedVerrucomicrobiain the marine environment. Here we reconstructed two genomes of symbioticVerrucomicrobiafrom bacterial metagenomes derived from the Atlanto-Mediterranean spongePetrosia ficiformisand three genomes from strains that we isolated from offshore seawater of the Eastern Mediterranean Sea. Phylogenomic analysis of these five strains indicated that they are all members ofVerrucomicrobiasubdivision 4, orderOpitutales. We compared these novel sponge-associated and seawater-isolated genomes to closely relatedVerrucomicrobia. Genomic analysis revealed thatPlanctomycetes-Verrucomicrobiamicrocompartment gene clusters are enriched in the genomes of symbioticOpitutalesincluding sponge symbionts but not in free-living ones. We hypothesize that in sponge symbionts these microcompartments are used for degradation ofl-fucose andl-rhamnose, which are components of algal and bacterial cell walls and therefore may be found at high concentrations in the sponge tissue. Furthermore, we observed an enrichment of toxin-antitoxin modules in symbioticOpitutales. We suggest that, in sponges, verrucomicrobial symbionts utilize these modules as a defence mechanism against antimicrobial activity deriving from the abundant microbial community co-inhabiting the host.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Microbiology

ISSN

1462-2912

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

20

Strana od-do

4669-4688

Kód UT WoS článku

000568333700001

EID výsledku v databázi Scopus

2-s2.0-85090777515