Raman spectroscopic search for scytonemin and gloeocapsin in endolithic colonizations in large gypsum crystals.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00552496" target="_blank" >RIV/60077344:_____/21:00552496 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11310/21:10436213
Výsledek na webu
<a href="https://doi.org/10.1002/jrs.6186" target="_blank" >https://doi.org/10.1002/jrs.6186</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jrs.6186" target="_blank" >10.1002/jrs.6186</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Raman spectroscopic search for scytonemin and gloeocapsin in endolithic colonizations in large gypsum crystals.
Popis výsledku v původním jazyce
Rock-dwelling microorganisms are frequently exposed to intense UV-radiation, and thus, they synthesize specialized UV-protective pigments. In this study, Raman microspectrometry was used for analysis of noncarotenoid UV-protective pigments of dark-pigmented endolithic colonization found in gypsum outcrops from three areas (Sicily, Poland and Israel). Samples were investigated using 445-, 532- and 780-nm excitation lasers. Two noncarotenoid UV-protective pigments scytonemin and gloeocapsin were detected in all studied sites. Major Raman bands of scytonemin were found at around 1593, 1552, 1438 and 1173 cm(-1). Gloeocapsin shows characteristic Raman bands similar to anthraquinone-based parietin of lichens: at 1665, 1575, 1378, 1310 and 465 cm(-1). Scytonemin and gloeocapsin are highly specific for cyanobacteria and therefore can be used as biomarkers of certain taxa of cyanobacteria. Detection of such pigments by Raman spectroscopy using three excitation wavelengths allows to gather more information about the composition of endolithic consortia using relatively fast and noninvasive methods and in their natural habitats.
Název v anglickém jazyce
Raman spectroscopic search for scytonemin and gloeocapsin in endolithic colonizations in large gypsum crystals.
Popis výsledku anglicky
Rock-dwelling microorganisms are frequently exposed to intense UV-radiation, and thus, they synthesize specialized UV-protective pigments. In this study, Raman microspectrometry was used for analysis of noncarotenoid UV-protective pigments of dark-pigmented endolithic colonization found in gypsum outcrops from three areas (Sicily, Poland and Israel). Samples were investigated using 445-, 532- and 780-nm excitation lasers. Two noncarotenoid UV-protective pigments scytonemin and gloeocapsin were detected in all studied sites. Major Raman bands of scytonemin were found at around 1593, 1552, 1438 and 1173 cm(-1). Gloeocapsin shows characteristic Raman bands similar to anthraquinone-based parietin of lichens: at 1665, 1575, 1378, 1310 and 465 cm(-1). Scytonemin and gloeocapsin are highly specific for cyanobacteria and therefore can be used as biomarkers of certain taxa of cyanobacteria. Detection of such pigments by Raman spectroscopy using three excitation wavelengths allows to gather more information about the composition of endolithic consortia using relatively fast and noninvasive methods and in their natural habitats.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA21-03322S" target="_blank" >GA21-03322S: Detekce biomarkerů endolitů evaporitických hornin: pozemský trénink pro exobiologii Marsu</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Raman Spectroscopy
ISSN
0377-0486
e-ISSN
1097-4555
Svazek periodika
52
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
15
Strana od-do
2633-2647
Kód UT WoS článku
000673570300001
EID výsledku v databázi Scopus
2-s2.0-85110318040