Genome analysis of Candida subhashii reveals its hybrid nature and dual mitochondrial genome conformations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00559976" target="_blank" >RIV/60077344:_____/21:00559976 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/dnaresearch/article/28/3/dsab006/6299387?login=true" target="_blank" >https://academic.oup.com/dnaresearch/article/28/3/dsab006/6299387?login=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/dnares/dsab006" target="_blank" >10.1093/dnares/dsab006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genome analysis of Candida subhashii reveals its hybrid nature and dual mitochondrial genome conformations

Popis výsledku v původním jazyce

Candida subhashii belongs to the CUG-Ser Glade, a group of phylogenetically closely related yeast species that includes some human opportunistic pathogens, such as Candida albicans. Despite being present in the environment, C. subhashii was initially described as the causative agent of a case of peritonitis. Considering the relevance of whole-genome sequencing and analysis for our understanding of genome evolution and pathogenicity, we sequenced, assembled and annotated the genome of C. subhashii type strain. Our results show that C. subhashii presents a highly heterozygous genome and other signatures that point to a hybrid ancestry. The presence of functional pathways for assimilation of hydroxyaromatic compounds goes in line with the affiliation of this yeast with soil microbial communities involved in lignin decomposition. Furthermore, we observed that different clones of this strain may present circular or linear mitochondrial DNA. Re-sequencing and comparison of strains with differential mitochondrial genome topology revealed five candidate genes potentially associated with this conformational change: MSK1, SSZ1, ALG5, MRPL9 and OYE32.

Název v anglickém jazyce

Genome analysis of Candida subhashii reveals its hybrid nature and dual mitochondrial genome conformations

Popis výsledku anglicky

Candida subhashii belongs to the CUG-Ser Glade, a group of phylogenetically closely related yeast species that includes some human opportunistic pathogens, such as Candida albicans. Despite being present in the environment, C. subhashii was initially described as the causative agent of a case of peritonitis. Considering the relevance of whole-genome sequencing and analysis for our understanding of genome evolution and pathogenicity, we sequenced, assembled and annotated the genome of C. subhashii type strain. Our results show that C. subhashii presents a highly heterozygous genome and other signatures that point to a hybrid ancestry. The presence of functional pathways for assimilation of hydroxyaromatic compounds goes in line with the affiliation of this yeast with soil microbial communities involved in lignin decomposition. Furthermore, we observed that different clones of this strain may present circular or linear mitochondrial DNA. Re-sequencing and comparison of strains with differential mitochondrial genome topology revealed five candidate genes potentially associated with this conformational change: MSK1, SSZ1, ALG5, MRPL9 and OYE32.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Dna Research

ISSN

1340-2838

e-ISSN

1756-1663

Svazek periodika

28

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

9

Strana od-do

dsab006

Kód UT WoS článku

000743559800002

EID výsledku v databázi Scopus

2-s2.0-85112486695