Another plea for 'best practice' in molecular approaches to trematode systematics: Diplostomum sp. clade Q identified as Diplostomum baeri Dubois, 1937 in Europe

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00556510" target="_blank" >RIV/60077344:_____/22:00556510 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/22:43920988

Výsledek na webu

<a href="https://www.cambridge.org/core/journals/parasitology/article/abs/another-plea-for-best-practice-in-molecular-approaches-to-trematode-systematics-diplostomum-sp-clade-q-identified-as-diplostomum-baeri-dubois-1937-in-europe/9E3EEFFE5DCF681D06F2671E2BFE42FB" target="_blank" >https://www.cambridge.org/core/journals/parasitology/article/abs/another-plea-for-best-practice-in-molecular-approaches-to-trematode-systematics-diplostomum-sp-clade-q-identified-as-diplostomum-baeri-dubois-1937-in-europe/9E3EEFFE5DCF681D06F2671E2BFE42FB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0031182021002092" target="_blank" >10.1017/S0031182021002092</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Another plea for 'best practice' in molecular approaches to trematode systematics: Diplostomum sp. clade Q identified as Diplostomum baeri Dubois, 1937 in Europe

Popis výsledku v původním jazyce

DNA sequence data became an integral part of species characterization and identification. Still, specimens associated with a particular DNA sequence must be identified by the use of traditional morphology-based analysis and correct linking of sequence and identification must be ensured. Only a small part of DNA sequences of the genus Diplostomum (Diplostomidae) is based on adult isolates which are essential for accurate identification. In this study, we provide species identification with an aid of morphological and molecular (cox1, ITS-5.8S-ITS2 and 28S) characterization of adults of Diplostomum baeri Dubois, 1937 from naturally infected Larus canus Linnaeus in Karelia, Russia. Furthermore, we reveal that the DNA sequences of our isolates of D. baeri are identical with those of the lineage Diplostomum sp. clade Q , while other sequences labelled as the 'D. baeri' complex do not represent lineages of D. baeri. Our new material of cercariae from Radix balthica (Linnaeus) in Ireland is also linked to Diplostomum sp. clade Q. We reveal that D. baeri is widely distributed in Europe, as first intermediate hosts lymnaeid snails (Radix auricularia (Linnaeus), R. balthica) are used, metacercariae occur in eye lens of cyprinid fishes. In light of the convoluted taxonomy of D. baeri and other Diplostomum spp., we extend the recommendations of Blasco-Costa et al. (2016, Systematic Parasitology 93, 295-306) for the 'best practice' in molecular approaches to trematode systematics. The current study is another step in elucidating the species spectrum of Diplostomum based on integrative taxonomy with well-described morphology of adults linked to sequences.

Název v anglickém jazyce

Another plea for 'best practice' in molecular approaches to trematode systematics: Diplostomum sp. clade Q identified as Diplostomum baeri Dubois, 1937 in Europe

Popis výsledku anglicky

DNA sequence data became an integral part of species characterization and identification. Still, specimens associated with a particular DNA sequence must be identified by the use of traditional morphology-based analysis and correct linking of sequence and identification must be ensured. Only a small part of DNA sequences of the genus Diplostomum (Diplostomidae) is based on adult isolates which are essential for accurate identification. In this study, we provide species identification with an aid of morphological and molecular (cox1, ITS-5.8S-ITS2 and 28S) characterization of adults of Diplostomum baeri Dubois, 1937 from naturally infected Larus canus Linnaeus in Karelia, Russia. Furthermore, we reveal that the DNA sequences of our isolates of D. baeri are identical with those of the lineage Diplostomum sp. clade Q , while other sequences labelled as the 'D. baeri' complex do not represent lineages of D. baeri. Our new material of cercariae from Radix balthica (Linnaeus) in Ireland is also linked to Diplostomum sp. clade Q. We reveal that D. baeri is widely distributed in Europe, as first intermediate hosts lymnaeid snails (Radix auricularia (Linnaeus), R. balthica) are used, metacercariae occur in eye lens of cyprinid fishes. In light of the convoluted taxonomy of D. baeri and other Diplostomum spp., we extend the recommendations of Blasco-Costa et al. (2016, Systematic Parasitology 93, 295-306) for the 'best practice' in molecular approaches to trematode systematics. The current study is another step in elucidating the species spectrum of Diplostomum based on integrative taxonomy with well-described morphology of adults linked to sequences.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/GA18-18597S" target="_blank" >GA18-18597S: Trematoda v potravních řetězcích subarktických jezer: vývoj kvantitativní základny a rámce pro výzkum ekologie společenstev v Arktidě</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology

ISSN

0031-1820

e-ISSN

1469-8161

Svazek periodika

149

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

503-518

Kód UT WoS článku

000740071700001

EID výsledku v databázi Scopus

2-s2.0-85122886259