Exploring cold hardiness within a butterfly clade: supercooling ability and polyol profiles in European Satyrinae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00557144" target="_blank" >RIV/60077344:_____/22:00557144 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/22:43904629

Výsledek na webu

<a href="https://www.mdpi.com/2075-4450/13/4/369/pdf?version=1649484990" target="_blank" >https://www.mdpi.com/2075-4450/13/4/369/pdf?version=1649484990</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/insects13040369" target="_blank" >10.3390/insects13040369</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exploring cold hardiness within a butterfly clade: supercooling ability and polyol profiles in European Satyrinae

Popis výsledku v původním jazyce

Simple Summary In insects distributed in temperate and cold zones, cold hardiness during overwintering crucially affects the distribution, including range shifts due to climate change. Our previous work on the genus Erebia, a cold-adapted and species-rich group of the sub-family Satyrinae (Nymphalidae), disclosed unexpected diversity of cold hardiness strategies, with closely related species surviving or not surviving freezing of larval body fluids. Asking whether this diversity is peculiar to this genus, or may be common in the Satyrinae clade, we investigated supercooling ability, contents of sugars and polyols in overwintering larvae tissues, and evolutionary signal of these traits of eight European Satyrinae species (from seven genera) and compared them with the Erebia representatives investigated earlier. We show that cold hardiness strategies are indeed diverse in the group and that high mountain and continental steppe species employ similar cryoprotection mechanisms, differing from those employed by species of more mesic environments. The cold hardiness of overwintering stages affects the distribution of temperate and cold-zone insects. Studies on Erebia, a species-rich cold-zone butterfly genus, detected unexpected diversity of cold hardiness traits. We expanded our investigation to eight Satyrinae species of seven genera. We assessed Autumn and Winter supercooling points (SCPs) and concentrations of putatively cryoprotective sugars and polyols via gas chromatography-mass spectrometry. Aphantopus hyperantus and Hipparchia semele survived freezing of body fluids, Coenonympha arcania, C. gardetta, and Melanargia galathea died prior to freezing, Maniola jurtina, Chazara briseis, and Minois dryas displayed a mixed response. SCP varied from -22 to -9 degrees C among species. Total sugar and polyol concentrations (TSPC) varied sixfold (2 to 12 mu g x mg(-1)) and eightfold including the Erebia spp. results. SCP and TSPC did not correlate. Alpine Erebia spp. contained high trehalose, threitol, and erythritol, C. briseis and C. gardetta contained high ribitol and trehalose, lowland species contained high saccharose, maltose, fructose, and sorbitol. SCP, TSPC, and glycerol concentrations were affected by phylogeny. Species of mountains or steppes tend to be freeze-avoidant, overwinter as young larvae, and contain high concentrations of trehalose, while those of mesic environments tend to be freeze-tolerant, overwinter as later instars, and rely on compounds such as maltose, saccharose, and fructose.

Název v anglickém jazyce

Exploring cold hardiness within a butterfly clade: supercooling ability and polyol profiles in European Satyrinae

Popis výsledku anglicky

Simple Summary In insects distributed in temperate and cold zones, cold hardiness during overwintering crucially affects the distribution, including range shifts due to climate change. Our previous work on the genus Erebia, a cold-adapted and species-rich group of the sub-family Satyrinae (Nymphalidae), disclosed unexpected diversity of cold hardiness strategies, with closely related species surviving or not surviving freezing of larval body fluids. Asking whether this diversity is peculiar to this genus, or may be common in the Satyrinae clade, we investigated supercooling ability, contents of sugars and polyols in overwintering larvae tissues, and evolutionary signal of these traits of eight European Satyrinae species (from seven genera) and compared them with the Erebia representatives investigated earlier. We show that cold hardiness strategies are indeed diverse in the group and that high mountain and continental steppe species employ similar cryoprotection mechanisms, differing from those employed by species of more mesic environments. The cold hardiness of overwintering stages affects the distribution of temperate and cold-zone insects. Studies on Erebia, a species-rich cold-zone butterfly genus, detected unexpected diversity of cold hardiness traits. We expanded our investigation to eight Satyrinae species of seven genera. We assessed Autumn and Winter supercooling points (SCPs) and concentrations of putatively cryoprotective sugars and polyols via gas chromatography-mass spectrometry. Aphantopus hyperantus and Hipparchia semele survived freezing of body fluids, Coenonympha arcania, C. gardetta, and Melanargia galathea died prior to freezing, Maniola jurtina, Chazara briseis, and Minois dryas displayed a mixed response. SCP varied from -22 to -9 degrees C among species. Total sugar and polyol concentrations (TSPC) varied sixfold (2 to 12 mu g x mg(-1)) and eightfold including the Erebia spp. results. SCP and TSPC did not correlate. Alpine Erebia spp. contained high trehalose, threitol, and erythritol, C. briseis and C. gardetta contained high ribitol and trehalose, lowland species contained high saccharose, maltose, fructose, and sorbitol. SCP, TSPC, and glycerol concentrations were affected by phylogeny. Species of mountains or steppes tend to be freeze-avoidant, overwinter as young larvae, and contain high concentrations of trehalose, while those of mesic environments tend to be freeze-tolerant, overwinter as later instars, and rely on compounds such as maltose, saccharose, and fructose.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Insects

ISSN

2075-4450

e-ISSN

2075-4450

Svazek periodika

13

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

369

Kód UT WoS článku

000786130700001

EID výsledku v databázi Scopus

2-s2.0-85128715821