Insect cross-tolerance to freezing and drought stress: role of metabolic rearrangement

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00558034" target="_blank" >RIV/60077344:_____/22:00558034 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/22:43904721

Výsledek na webu

<a href="https://royalsocietypublishing.org/doi/10.1098/rspb.2022.0308" target="_blank" >https://royalsocietypublishing.org/doi/10.1098/rspb.2022.0308</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1098/rspb.2022.0308" target="_blank" >10.1098/rspb.2022.0308</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Insect cross-tolerance to freezing and drought stress: role of metabolic rearrangement

Popis výsledku v původním jazyce

The accumulation of trehalose has been suggested as a mechanism underlying insect cross-tolerance to cold/freezing and drought. Here we show that exposing diapausing larvae of the drosophilid fly, Chymomyza costata to dry conditions significantly stimulates their freeze tolerance. It does not, however, improve their tolerance to desiccation, nor does it significantly affect trehalose concentrations. Next, we use metabolomics to compare the complex alterations to intermediary metabolism pathways in response to three environmental factors with different ecological meanings: environmental drought (an environmental stressor causing mortality), decreasing ambient temperatures (an acclimation stimulus for improvement of cold hardiness), and short days (an environmental signal inducing diapause). We show that all three factors trigger qualitatively similar metabolic rearrangement and a similar phenotypic outcome - improved larval freeze tolerance. The similarities in metabolic response include (but are not restricted to) the accumulation of typical compatible solutes and the accumulation of energy-rich molecules (phosphagens). Based on these results, we suggest that transition to metabolic suppression (a state in which chemical energy demand is relatively low but need for stabilization of macromolecules is high) represents a common axis of metabolic pathway reorganization towards accumulation of non-toxic cytoprotective compounds, which in turn stimulates larval freeze tolerance.

Název v anglickém jazyce

Insect cross-tolerance to freezing and drought stress: role of metabolic rearrangement

Popis výsledku anglicky

The accumulation of trehalose has been suggested as a mechanism underlying insect cross-tolerance to cold/freezing and drought. Here we show that exposing diapausing larvae of the drosophilid fly, Chymomyza costata to dry conditions significantly stimulates their freeze tolerance. It does not, however, improve their tolerance to desiccation, nor does it significantly affect trehalose concentrations. Next, we use metabolomics to compare the complex alterations to intermediary metabolism pathways in response to three environmental factors with different ecological meanings: environmental drought (an environmental stressor causing mortality), decreasing ambient temperatures (an acclimation stimulus for improvement of cold hardiness), and short days (an environmental signal inducing diapause). We show that all three factors trigger qualitatively similar metabolic rearrangement and a similar phenotypic outcome - improved larval freeze tolerance. The similarities in metabolic response include (but are not restricted to) the accumulation of typical compatible solutes and the accumulation of energy-rich molecules (phosphagens). Based on these results, we suggest that transition to metabolic suppression (a state in which chemical energy demand is relatively low but need for stabilization of macromolecules is high) represents a common axis of metabolic pathway reorganization towards accumulation of non-toxic cytoprotective compounds, which in turn stimulates larval freeze tolerance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

<a href="/cs/project/GA19-13381S" target="_blank" >GA19-13381S: Kryoprotektivní látky a jejich účinek: testování cílových molekul odvozených z výzkumu mrazuvzdorné octomilky.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proceedings of the Royal Society B-Biological Sciences

ISSN

0962-8452

e-ISSN

1471-2954

Svazek periodika

289

Číslo periodika v rámci svazku

JUN 8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

20220308

Kód UT WoS článku

000807984900013

EID výsledku v databázi Scopus

2-s2.0-85131475074