Ecogenomics sheds light on diverse lifestyle strategies in freshwater CPR.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00558631" target="_blank" >RIV/60077344:_____/22:00558631 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/22:43904957

Výsledek na webu

<a href="https://doi.org/10.1186/s40168-022-01274-3" target="_blank" >https://doi.org/10.1186/s40168-022-01274-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s40168-022-01274-3" target="_blank" >10.1186/s40168-022-01274-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ecogenomics sheds light on diverse lifestyle strategies in freshwater CPR.

Popis výsledku v původním jazyce

Background: The increased use of metagenomics and single-cell genomics led to the discovery of organisms from phyla with no cultivated representatives and proposed new microbial lineages such as the candidate phyla radiation (CPR or Patescibacteria). These bacteria have peculiar ribosomal structures, reduced metabolic capacities, small genome, and cell sizes, and a general host-associated lifestyle was proposed for the radiation. So far, most CPR genomes were obtained from groundwaters., however, their diversity, abundance, and role in surface freshwaters is largely unexplored. Here, we attempt to close these knowledge gaps by deep metagenomic sequencing of 119 samples of 17 different freshwater lakes located in Europe and Asia. Moreover, we applied Fluorescence in situ Hybridization followed by Catalyzed Reporter Deposition (CARD-FISH) for a first visualization of distinct CPR lineages in freshwater samples.

Název v anglickém jazyce

Ecogenomics sheds light on diverse lifestyle strategies in freshwater CPR.

Popis výsledku anglicky

Background: The increased use of metagenomics and single-cell genomics led to the discovery of organisms from phyla with no cultivated representatives and proposed new microbial lineages such as the candidate phyla radiation (CPR or Patescibacteria). These bacteria have peculiar ribosomal structures, reduced metabolic capacities, small genome, and cell sizes, and a general host-associated lifestyle was proposed for the radiation. So far, most CPR genomes were obtained from groundwaters., however, their diversity, abundance, and role in surface freshwaters is largely unexplored. Here, we attempt to close these knowledge gaps by deep metagenomic sequencing of 119 samples of 17 different freshwater lakes located in Europe and Asia. Moreover, we applied Fluorescence in situ Hybridization followed by Catalyzed Reporter Deposition (CARD-FISH) for a first visualization of distinct CPR lineages in freshwater samples.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbiome

ISSN

2049-2618

e-ISSN

2049-2618

Svazek periodika

10

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

21

Strana od-do

84

Kód UT WoS článku

000805790100001

EID výsledku v databázi Scopus

2-s2.0-85131457129