Abolition of egg diapause by ablation of suboesophageal ganglion in parental females is compatible with genetic engineering methods
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00561680" target="_blank" >RIV/60077344:_____/22:00561680 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60076658:12310/22:43905095
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0022191022000841?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0022191022000841?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jinsphys.2022.104438" target="_blank" >10.1016/j.jinsphys.2022.104438</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Abolition of egg diapause by ablation of suboesophageal ganglion in parental females is compatible with genetic engineering methods
Popis výsledku v původním jazyce
Microinjection of genetic material into non-diapause eggs is required for genetic engineering of silkworms. Besides diapause could be useful for maintaining transgenic lines, a drawback of this technology is that most standard silkworm strains and experimental lines of interest produce diapausing eggs. Several approaches have been developed to abolish diapause but none are very efficient. Here, we investigated the ablation of the suboesophageal ganglion (SG) in female pupae, which is a source of the hormone required to trigger egg diapause, as a mean to abolish diapause. We showed that SG-ablation is a reliable method to produce nondiapause eggs. Additionally, the challenge associated with lower fecundity of females with SG ablation was resolved by injecting pilocarpine into the mated female. We also investigated the suitability of nondiapause eggs laid by SG-ablated females for transgenesis, targeted mutagenesis, and induction of parthenogenetic development. Our results demonstrated SG-ablation to be a useful and simple method for expanding the possibilities associated with genetic engineering in silkworms.
Název v anglickém jazyce
Abolition of egg diapause by ablation of suboesophageal ganglion in parental females is compatible with genetic engineering methods
Popis výsledku anglicky
Microinjection of genetic material into non-diapause eggs is required for genetic engineering of silkworms. Besides diapause could be useful for maintaining transgenic lines, a drawback of this technology is that most standard silkworm strains and experimental lines of interest produce diapausing eggs. Several approaches have been developed to abolish diapause but none are very efficient. Here, we investigated the ablation of the suboesophageal ganglion (SG) in female pupae, which is a source of the hormone required to trigger egg diapause, as a mean to abolish diapause. We showed that SG-ablation is a reliable method to produce nondiapause eggs. Additionally, the challenge associated with lower fecundity of females with SG ablation was resolved by injecting pilocarpine into the mated female. We also investigated the suitability of nondiapause eggs laid by SG-ablated females for transgenesis, targeted mutagenesis, and induction of parthenogenetic development. Our results demonstrated SG-ablation to be a useful and simple method for expanding the possibilities associated with genetic engineering in silkworms.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10605 - Developmental biology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Insect Physiology
ISSN
0022-1910
e-ISSN
1879-1611
Svazek periodika
142
Číslo periodika v rámci svazku
OCT 01
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
9
Strana od-do
104438
Kód UT WoS článku
000855581600003
EID výsledku v databázi Scopus
2-s2.0-85137409697