Repeat-based holocentromeres influence genome architecture and karyotype evolution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00568618" target="_blank" >RIV/60077344:_____/22:00568618 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/22:00568618 RIV/60076658:12310/22:43904991

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0092867422007978?via%3Dihub#kwrds0010" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0092867422007978?via%3Dihub#kwrds0010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cell.2022.06.045" target="_blank" >10.1016/j.cell.2022.06.045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Repeat-based holocentromeres influence genome architecture and karyotype evolution

Popis výsledku v původním jazyce

The centromere represents a single region in most eukaryotic chromosomes. However, several plant and animal lineages assemble holocentromeres along the entire chromosome length. Here, we compare genome organization and evolution as a function of centromere type by assembling chromosome-scale hol-ocentric genomes with repeat-based holocentromeres from three beak-sedge (Rhynchospora pubera, R. breviuscula, and R. tenuis) and their closest monocentric relative, Juncus effusus. We demonstrate that transition to holocentricity affected 3D genome architecture by redefining genomic compartments, while distributing centromere function to thousands of repeat-based centromere units genome-wide. We uncover a complex genome organization in R. pubera that hides its unexpected octoploidy and describe a marked reduction in chromosome number for R. tenuis, which has only two chromosomes. We show that chromo-some fusions, facilitated by repeat-based holocentromeres, promoted karyotype evolution and diploidiza-tion. Our study thus sheds light on several important aspects of genome architecture and evolution influenced by centromere organization.

Název v anglickém jazyce

Repeat-based holocentromeres influence genome architecture and karyotype evolution

Popis výsledku anglicky

The centromere represents a single region in most eukaryotic chromosomes. However, several plant and animal lineages assemble holocentromeres along the entire chromosome length. Here, we compare genome organization and evolution as a function of centromere type by assembling chromosome-scale hol-ocentric genomes with repeat-based holocentromeres from three beak-sedge (Rhynchospora pubera, R. breviuscula, and R. tenuis) and their closest monocentric relative, Juncus effusus. We demonstrate that transition to holocentricity affected 3D genome architecture by redefining genomic compartments, while distributing centromere function to thousands of repeat-based centromere units genome-wide. We uncover a complex genome organization in R. pubera that hides its unexpected octoploidy and describe a marked reduction in chromosome number for R. tenuis, which has only two chromosomes. We show that chromo-some fusions, facilitated by repeat-based holocentromeres, promoted karyotype evolution and diploidiza-tion. Our study thus sheds light on several important aspects of genome architecture and evolution influenced by centromere organization.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell

ISSN

0092-8674

e-ISSN

1097-4172

Svazek periodika

185

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

3153-3168

Kód UT WoS článku

000877930800003

EID výsledku v databázi Scopus

2-s2.0-85135412549