Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00569079" target="_blank" >RIV/60077344:_____/22:00569079 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/22:00558113 RIV/60076658:12310/22:43905006 RIV/00064203:_____/22:10444203 RIV/00216208:11130/22:10444203

Výsledek na webu

<a href="https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220019/parasite220019.html" target="_blank" >https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220019/parasite220019.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1051/parasite/2022029" target="_blank" >10.1051/parasite/2022029</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans

Popis výsledku v původním jazyce

Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR) and qPCR in a set of 288 DNA samples obtained from stool samples of gut-healthy individuals, and (ii) subtype diversity as detected by next-generation sequencing (NGS) versus Sanger sequencing. Real-time PCR resulted in more positive samples than cPCR, revealing high fecal load of Blastocystis based on the quantification curve in most samples. In subtype detection, NGS was largely in agreement with Sanger sequencing but showed higher sensitivity for mixed subtype colonization within one host. This fact together with use of the combination of qPCR and NGS and obtaining information on the fecal protist load will be beneficial for epidemiological and surveillance studies.

Název v anglickém jazyce

Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans

Popis výsledku anglicky

Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR) and qPCR in a set of 288 DNA samples obtained from stool samples of gut-healthy individuals, and (ii) subtype diversity as detected by next-generation sequencing (NGS) versus Sanger sequencing. Real-time PCR resulted in more positive samples than cPCR, revealing high fecal load of Blastocystis based on the quantification curve in most samples. In subtype detection, NGS was largely in agreement with Sanger sequencing but showed higher sensitivity for mixed subtype colonization within one host. This fact together with use of the combination of qPCR and NGS and obtaining information on the fecal protist load will be beneficial for epidemiological and surveillance studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30310 - Parasitology

Projekt

<a href="/cs/project/GA22-04837S" target="_blank" >GA22-04837S: Vztahy mezi prvoky a bakteriomem ve střevním ekosystému domácích psů a jejich majitelů při přechodu na moderní životní styl</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasite

ISSN

1252-607X

e-ISSN

1776-1042

Svazek periodika

29

Číslo periodika v rámci svazku

MAY

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

7

Strana od-do

30

Kód UT WoS článku

000803306000001

EID výsledku v databázi Scopus

2-s2.0-85131203128