A novel membrane complex is required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00570594" target="_blank" >RIV/60077344:_____/22:00570594 - isvavai.cz</a>

Výsledek na webu

<a href="https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010194" target="_blank" >https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010194</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pgen.1010194" target="_blank" >10.1371/journal.pgen.1010194</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A novel membrane complex is required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila

Popis výsledku v původním jazyce

Author summaryAll cells, whether single-celled protists or multicellular organisms, interact dynamically with their environments. One important mode of interaction is the release of molecules, a phenomenon called secretion, which can then modify the environment to promote the organism's well-being. Moreover, many cells have the capacity to rapidly adjust the pathways that underlie secretion, allowing them to tailor their secretory behavior in response to changes in their surroundings. A dramatic example of this is the capacity to synthesize and then store reservoirs of secretory molecules, whose eventual release is triggered when the cell senses specific environmental conditions. This phenomenon is called 'regulated exocytosis' and has been long studied in animals, because it serves as the basis for communication between different cells and tissues. Many single-celled organisms can also secrete via regulated exocytosis, and understanding the mechanisms involved could have practical consequences for developing therapies against several devastating human parasites. In this paper, we took a genetic approach to identifying factors involved in exocytosis in a single-celled protist, the ciliate Tetrahymena thermophila. We find that a novel gene, which appears to be present only in Tetrahymena and its relatively close evolutionary relatives, plays an important role in the pathway. Our results add another layer to recent findings that cells like Tetrahymena evolved unique mechanisms for regulated exocytosis, expanding our appreciation of cellular biodiversity.

Název v anglickém jazyce

A novel membrane complex is required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila

Popis výsledku anglicky

Author summaryAll cells, whether single-celled protists or multicellular organisms, interact dynamically with their environments. One important mode of interaction is the release of molecules, a phenomenon called secretion, which can then modify the environment to promote the organism's well-being. Moreover, many cells have the capacity to rapidly adjust the pathways that underlie secretion, allowing them to tailor their secretory behavior in response to changes in their surroundings. A dramatic example of this is the capacity to synthesize and then store reservoirs of secretory molecules, whose eventual release is triggered when the cell senses specific environmental conditions. This phenomenon is called 'regulated exocytosis' and has been long studied in animals, because it serves as the basis for communication between different cells and tissues. Many single-celled organisms can also secrete via regulated exocytosis, and understanding the mechanisms involved could have practical consequences for developing therapies against several devastating human parasites. In this paper, we took a genetic approach to identifying factors involved in exocytosis in a single-celled protist, the ciliate Tetrahymena thermophila. We find that a novel gene, which appears to be present only in Tetrahymena and its relatively close evolutionary relatives, plays an important role in the pathway. Our results add another layer to recent findings that cells like Tetrahymena evolved unique mechanisms for regulated exocytosis, expanding our appreciation of cellular biodiversity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/EF16_019%2F0000759" target="_blank" >EF16_019/0000759: Centrum výzkumu patogenity a virulence parazitů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS Genetics

ISSN

1553-7404

e-ISSN

1553-7404

Svazek periodika

18

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

33

Strana od-do

e1010194

Kód UT WoS článku

000944865500016

EID výsledku v databázi Scopus

2-s2.0-85131268578