Physiological responses to honeybee venom poisoning in a model organism, the firebug Pyrrhocoris apterus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00572215" target="_blank" >RIV/60077344:_____/23:00572215 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/23:43907281 RIV/60076658:12410/23:43907281 RIV/61989592:15310/23:73622307

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1532045623001126/pdfft?md5=f4f8981fef7d854382ef1fb98ada3b26&pid=1-s2.0-S1532045623001126-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1532045623001126/pdfft?md5=f4f8981fef7d854382ef1fb98ada3b26&pid=1-s2.0-S1532045623001126-main.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbpc.2023.109657" target="_blank" >10.1016/j.cbpc.2023.109657</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Physiological responses to honeybee venom poisoning in a model organism, the firebug Pyrrhocoris apterus

Popis výsledku v původním jazyce

In this study, the biochemical and physiological features of the firebug Pyrrhocoris apterus were investigated to understand the impact of the honeybee Apis mellifera venom on them using physiological methods (mortality, total level of metabolism), biochemical methods (ELISA, mass spectrometry, polyacrylamide gel electrophoresis, spectrophotometry) and molecular methods (real-time PCR). Together, the obtained findings suggest that venom injection increased the level of adipokinetic hormone (AKH) in the CNS of P. apterus, indicating that this hormone plays a key role in activating defence responses. Furthermore, histamine levels in the gut increased significantly after envenomation and did not seem to be modulated by AKH. In contrast, histamine levels in the haemolymph increased after treatment with AKH and AKH + venom. In addition, we found that vitellogenin levels in haemolymph decreased in both males and females after venom application. Lipids, which are the main energy metabolites used by Pyrrhocoris, were significantly exhausted from the haemolymph after the administration of venom and the co-application with AKH reversed this effect. However, we did not find much influence on the effect of digestive enzymes after the injection of venom. Our research has highlighted the noticeable effect of bee venom on P. apterus’ body and provided new insights into the role of AKH in controlling defensive responses. However, it is also likely that there will be alternative defence mechanisms.

Název v anglickém jazyce

Physiological responses to honeybee venom poisoning in a model organism, the firebug Pyrrhocoris apterus

Popis výsledku anglicky

In this study, the biochemical and physiological features of the firebug Pyrrhocoris apterus were investigated to understand the impact of the honeybee Apis mellifera venom on them using physiological methods (mortality, total level of metabolism), biochemical methods (ELISA, mass spectrometry, polyacrylamide gel electrophoresis, spectrophotometry) and molecular methods (real-time PCR). Together, the obtained findings suggest that venom injection increased the level of adipokinetic hormone (AKH) in the CNS of P. apterus, indicating that this hormone plays a key role in activating defence responses. Furthermore, histamine levels in the gut increased significantly after envenomation and did not seem to be modulated by AKH. In contrast, histamine levels in the haemolymph increased after treatment with AKH and AKH + venom. In addition, we found that vitellogenin levels in haemolymph decreased in both males and females after venom application. Lipids, which are the main energy metabolites used by Pyrrhocoris, were significantly exhausted from the haemolymph after the administration of venom and the co-application with AKH reversed this effect. However, we did not find much influence on the effect of digestive enzymes after the injection of venom. Our research has highlighted the noticeable effect of bee venom on P. apterus’ body and provided new insights into the role of AKH in controlling defensive responses. However, it is also likely that there will be alternative defence mechanisms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LUAUS23128" target="_blank" >LUAUS23128: Studium vlivu klimatických změn na stav včelstev prostřednictvím biologických analýz a umělé inteligence.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Comparative Biochemistry and Physiology Part C

ISSN

1532-0456

e-ISSN

1878-1659

Svazek periodika

270

Číslo periodika v rámci svazku

AUG

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

109657

Kód UT WoS článku

001054835700001

EID výsledku v databázi Scopus

2-s2.0-85159597604