Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00572378" target="_blank" >RIV/60077344:_____/23:00572378 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14310/23:00132146 RIV/62157124:16270/23:43881026
Výsledek na webu
<a href="https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011038" target="_blank" >https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011038</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pntd.0011038" target="_blank" >10.1371/journal.pntd.0011038</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid
Popis výsledku v původním jazyce
Author summaryA potentially fatal disease, neuroangiostrongyliasis, is caused by the rat lungworm (Angiostrongylus cantonensis). The parasite migrates into the spinal cord and brain of accidental hosts, such as humans and dogs, after ingestion of infective larvae, for example by eating snails in garden fruit or vegetables. Recently, an ultrasensitive molecular assay which can detect tiny fragments of the parasite's DNA was developed and has been used to establish a diagnosis. Although this assay outperforms previously developed assays, it requires clean DNA with specialised equipment in a laboratory setting. There is an urgent need for an alternative diagnostic method which is sensitive and portable, for deployment in the field and in the hospitals in remote areas or in low-income countries. The authors developed a fast and portable loop-mediated isothermal amplification (LAMP) assay that compares favourably to the ultra-sensitive PCR assay when tested using cerebrospinal fluid from dogs on the Australian east coast with presumptive neuroangiostrongyliasis. Considering a 'One Health' approach to diagnostics, this assay enables portable emergency diagnostics equally suitable to humans, dogs and wildlife. The newly developed assay will also enable water supplies to be screened, as well as crustaceans and molluscs used as potential food sources, for presence of the parasite.
Název v anglickém jazyce
Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid
Popis výsledku anglicky
Author summaryA potentially fatal disease, neuroangiostrongyliasis, is caused by the rat lungworm (Angiostrongylus cantonensis). The parasite migrates into the spinal cord and brain of accidental hosts, such as humans and dogs, after ingestion of infective larvae, for example by eating snails in garden fruit or vegetables. Recently, an ultrasensitive molecular assay which can detect tiny fragments of the parasite's DNA was developed and has been used to establish a diagnosis. Although this assay outperforms previously developed assays, it requires clean DNA with specialised equipment in a laboratory setting. There is an urgent need for an alternative diagnostic method which is sensitive and portable, for deployment in the field and in the hospitals in remote areas or in low-income countries. The authors developed a fast and portable loop-mediated isothermal amplification (LAMP) assay that compares favourably to the ultra-sensitive PCR assay when tested using cerebrospinal fluid from dogs on the Australian east coast with presumptive neuroangiostrongyliasis. Considering a 'One Health' approach to diagnostics, this assay enables portable emergency diagnostics equally suitable to humans, dogs and wildlife. The newly developed assay will also enable water supplies to be screened, as well as crustaceans and molluscs used as potential food sources, for presence of the parasite.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30303 - Infectious Diseases
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS Neglected Tropical Diseases
ISSN
1935-2735
e-ISSN
1935-2735
Svazek periodika
17
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
14
Strana od-do
e0011038
Kód UT WoS článku
000984025300006
EID výsledku v databázi Scopus
2-s2.0-85159740412