Characterisation of the photosynthetic complexes from the marine gammaproteobacterium Congregibacter litoralis KT71

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00572692" target="_blank" >RIV/60077344:_____/23:00572692 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/23:00572692 RIV/60076658:12310/23:43907196

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0005272822004169?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0005272822004169?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbabio.2022.148946" target="_blank" >10.1016/j.bbabio.2022.148946</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of the photosynthetic complexes from the marine gammaproteobacterium Congregibacter litoralis KT71

Popis výsledku v původním jazyce

Possibly the most abundant group of anoxygenic phototrophs are marine photoheterotrophic Gammaproteobac-teria belonging to the NOR5/OM60 clade. As little is known about their photosynthetic apparatus, the photo-synthetic complexes from the marine phototrophic bacterium Congregibacter litoralis KT71 were purified and spectroscopically characterised. The intra-cytoplasmic membranes contain a smaller amount of photosynthetic complexes when compared with anaerobic purple bacteria. Moreover, the intra-cytoplasmic membranes contain only a minimum amount of peripheral LH2 complexes. The complexes are populated by bacteriochlorophyll a, spirilloxanthin and two novel ketocarotenoids, with biophysical and biochemical properties similar to previously characterised complexes from purple bacteria. The organization of the RC-LH1 complex has been further char-acterised using cryo-electron microscopy. The overall organisation is similar to the complex from the gam-maproteobacterium Thermochromatium tepidum, with the type-II reaction centre surrounded by a slightly elliptical LH1 antenna ring composed of 16 alpha beta-subunits with no discernible gap or pore. The RC-LH1 and LH2 apoproteins are phylogenetically related to other halophilic species but LH2 also to some alphaproteobacterial species. It seems that the reduction of light-harvesting apparatus and acquisition of novel ketocarotenoids in Congregibacter litoralis KT71 represent specific adaptations for operating the anoxygenic photosynthesis under aerobic conditions at sea.

Název v anglickém jazyce

Characterisation of the photosynthetic complexes from the marine gammaproteobacterium Congregibacter litoralis KT71

Popis výsledku anglicky

Possibly the most abundant group of anoxygenic phototrophs are marine photoheterotrophic Gammaproteobac-teria belonging to the NOR5/OM60 clade. As little is known about their photosynthetic apparatus, the photo-synthetic complexes from the marine phototrophic bacterium Congregibacter litoralis KT71 were purified and spectroscopically characterised. The intra-cytoplasmic membranes contain a smaller amount of photosynthetic complexes when compared with anaerobic purple bacteria. Moreover, the intra-cytoplasmic membranes contain only a minimum amount of peripheral LH2 complexes. The complexes are populated by bacteriochlorophyll a, spirilloxanthin and two novel ketocarotenoids, with biophysical and biochemical properties similar to previously characterised complexes from purple bacteria. The organization of the RC-LH1 complex has been further char-acterised using cryo-electron microscopy. The overall organisation is similar to the complex from the gam-maproteobacterium Thermochromatium tepidum, with the type-II reaction centre surrounded by a slightly elliptical LH1 antenna ring composed of 16 alpha beta-subunits with no discernible gap or pore. The RC-LH1 and LH2 apoproteins are phylogenetically related to other halophilic species but LH2 also to some alphaproteobacterial species. It seems that the reduction of light-harvesting apparatus and acquisition of novel ketocarotenoids in Congregibacter litoralis KT71 represent specific adaptations for operating the anoxygenic photosynthesis under aerobic conditions at sea.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Bioenergetics

ISSN

0005-2728

e-ISSN

1879-2650

Svazek periodika

1864

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

148946

Kód UT WoS článku

000995442400001

EID výsledku v databázi Scopus

2-s2.0-85143117335