Xenorhabdus aichiensis sp. nov., Xenorhabdus anantnagensis sp. nov., and Xenorhabdus yunnanensis sp. nov., Isolated from Steinernema Entomopathogenic Nematodes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00574854" target="_blank" >RIV/60077344:_____/23:00574854 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/content/pdf/10.1007/s00284-023-03373-2.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007/s00284-023-03373-2.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00284-023-03373-2" target="_blank" >10.1007/s00284-023-03373-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Xenorhabdus aichiensis sp. nov., Xenorhabdus anantnagensis sp. nov., and Xenorhabdus yunnanensis sp. nov., Isolated from Steinernema Entomopathogenic Nematodes

Popis výsledku v původním jazyce

Three bacterial strains, XENO-2(T), XENO-7(T), and XENO-10(T), isolated from Steinernema entomopathogenic nematodes, were found to represent novel Xenorhabdus species. In this study, we describe these new species by whole-genome and whole-proteome phylogenomic reconstructions, by calculating sequence identity scores using core genome sequences, and by phenotypic characterization. Phylogenomic reconstructions using ribosomal and house-keeping genes, and whole-genome and whole-proteome sequences show that XENO-2(T) and XENO-10(T) are closely related to Xenorhabdus japonica DSM 16522(T) and that XENO-7(T) is closely related to Xenorhabdus bovienii subsp. africana XENO-1(T) and to X. bovienii subsp. bovienii T228(T). The dDDH values between XENO-2(T) and XENO-10(T) and between XENO-2(T) and X. japonica DSM 16522(T) are 56.4 and 51.8%, respectively. The dDDH value between XENO-10(T) and X. japonica DSM 16522(T) is 53.4%. The dDDH values between XENO-7(T) and X. bovienii subsp. africana XENO-1(T) and between XENO-7(T) and X. bovienii subsp. bovienii T228(T) are 63.6 and 69.4%, respectively. These dDDH values are below the 70% divergence threshold for prokaryotic species delineation. The newly described species are highly pathogenic to G. mellonella larvae, grow at pH between 5 and 9 (optimum 5-7), at salt concentrations of 1-3% (optimum 1-2%), and temperatures between 20 and 37 degrees C (optimum 28-30 degrees C). Biochemical tests such as lysine decarboxylase, ornithine decarboxylase, urease, gelatinase, citrate utilization, indole and acetoin production, and cytochrome oxidase tests allow to differentiate the novel species from their more closely related species. Considering these genetic and phenotypic divergencies, we propose the following new species: Xenorhabdus aichiensis sp. nov. with XENO-7(T) (= CCM 9233(T) = CCOS 2024(T)) as the type strain, Xenorhabdus anantnagensis sp. nov., with XENO-2(T) (= CCM 9237(T) = CCOS 2023(T)) as the type strain, and Xenorhabdus yunnanensis sp. nov., with XENO-10(T) (= CCM 9322(T) = CCOS 2071(T)) as the type strain. Our study contributes to a better understanding of the biodiversity and phylogenetic relationships of entomopathogenic bacteria associated with insect parasitic nematodes.

Název v anglickém jazyce

Xenorhabdus aichiensis sp. nov., Xenorhabdus anantnagensis sp. nov., and Xenorhabdus yunnanensis sp. nov., Isolated from Steinernema Entomopathogenic Nematodes

Popis výsledku anglicky

Three bacterial strains, XENO-2(T), XENO-7(T), and XENO-10(T), isolated from Steinernema entomopathogenic nematodes, were found to represent novel Xenorhabdus species. In this study, we describe these new species by whole-genome and whole-proteome phylogenomic reconstructions, by calculating sequence identity scores using core genome sequences, and by phenotypic characterization. Phylogenomic reconstructions using ribosomal and house-keeping genes, and whole-genome and whole-proteome sequences show that XENO-2(T) and XENO-10(T) are closely related to Xenorhabdus japonica DSM 16522(T) and that XENO-7(T) is closely related to Xenorhabdus bovienii subsp. africana XENO-1(T) and to X. bovienii subsp. bovienii T228(T). The dDDH values between XENO-2(T) and XENO-10(T) and between XENO-2(T) and X. japonica DSM 16522(T) are 56.4 and 51.8%, respectively. The dDDH value between XENO-10(T) and X. japonica DSM 16522(T) is 53.4%. The dDDH values between XENO-7(T) and X. bovienii subsp. africana XENO-1(T) and between XENO-7(T) and X. bovienii subsp. bovienii T228(T) are 63.6 and 69.4%, respectively. These dDDH values are below the 70% divergence threshold for prokaryotic species delineation. The newly described species are highly pathogenic to G. mellonella larvae, grow at pH between 5 and 9 (optimum 5-7), at salt concentrations of 1-3% (optimum 1-2%), and temperatures between 20 and 37 degrees C (optimum 28-30 degrees C). Biochemical tests such as lysine decarboxylase, ornithine decarboxylase, urease, gelatinase, citrate utilization, indole and acetoin production, and cytochrome oxidase tests allow to differentiate the novel species from their more closely related species. Considering these genetic and phenotypic divergencies, we propose the following new species: Xenorhabdus aichiensis sp. nov. with XENO-7(T) (= CCM 9233(T) = CCOS 2024(T)) as the type strain, Xenorhabdus anantnagensis sp. nov., with XENO-2(T) (= CCM 9237(T) = CCOS 2023(T)) as the type strain, and Xenorhabdus yunnanensis sp. nov., with XENO-10(T) (= CCM 9322(T) = CCOS 2071(T)) as the type strain. Our study contributes to a better understanding of the biodiversity and phylogenetic relationships of entomopathogenic bacteria associated with insect parasitic nematodes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Microbiology

ISSN

0343-8651

e-ISSN

1432-0991

Svazek periodika

80

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

16

Strana od-do

300

Kód UT WoS článku

001037726600005

EID výsledku v databázi Scopus

2-s2.0-85165680131